Histone deacetylase (HDAC) inhibitors have the potential to de-repress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. Dietary HDAC inhibitors derived from natural phytochemicals are promising anticancer agents. In this thesis, metabolites from natural organosulfur and organoselenium compounds, i.e. allyl mercaptan (AM), β-methylselenopyruvate (MSP) and...
Met is a prognostic indicator of colorectal cancer patient survival. Therapies that target Met may therefore have beneficial outcomes in the clinic. Recently, EGCG was reported to suppress Met activation, although the mechanisms were not elucidated. HCT116 and HT29 human colon cancer cells were used to examine the relationships between...
β-catenin is a multi functional protein that is involved in cell-cell adhesion
and cell signaling. In non-stimulated cells, β-catenin is tightly down-regulated by
GSK-3β-dependent phosphorylation at Ser and Thr residues, followed by rapid
ubiquitination and proteasomal degradation. It is well established that mutations
within the regulatory GSK-3β region lead to...
Targeting the epigenome, including the use of histone deacetylase (HDAC)
inhibitors, is a novel strategy for cancer chemoprevention. Sulforaphane
(SFN), a compound found at high levels in broccoli and broccoli sprouts, is a
potent inducer of Phase 2 detoxification enzymes and inhibits tumorigenesis
in animal models. SFN also has a...
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
Full Text:
Colon Cancer
Matthew Kaiser
Frei Lab
&
Dashwood Lab
“Epigenetics refers to changes in gene
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
Full Text:
Kaiser
Frei Lab
&
Dashwood Lab
“Epigenetics refers to changes in gene expression caused by mechanisms
The detection and determination of clusters has been of special interest among re-
searchers from different fields for a long time. In particular, assessing whether the clusters
are significant is a question that has been asked by a number of experimenters. In Fuentes
and Casella (2009), the authors put forth...
The antimutagenic potency of wheat grain and berry extracts was studied in
vitro against several heterocyclic amines (HCAs) using the Salmonella
mutagenicity assay and the anticarcinogencity of wheat grain was studied in vivo
using the rat colonic aberrant crypt focus assay.
Wheat bran, which binds HCAs in vitro, as well...