The advent of improved DNA sequencing technologies has allowed the analysis of various microbial communities. Bloom-forming freshwater cyanobacteria can produce toxins and taste-and-odor compounds that can negatively affect drinking water supplies. Here, I have employed second- and third-generation sequencing technologies to characterize bloom-forming freshwater cyanobacterial genomes and their associated heterotrophic...
Limnology is undergoing a transition to high-throughput -omic analysis of freshwater
bacterial communities. An important first step in making the transition is to
characterize several genomes that can be used as references to guide metagenome
assembly and analysis. Here I characterize four new freshwater cyanobacterial
genomes, a pair of lake...
Toxic
cyanobacterial
blooms
in
freshwater
sources
are
of
increasing
concern
due
to
the
production
of
toxins
that
pose
a
threat
to
human
health.
Both
toxic
and
non-toxic
strains
of
Microcystis
aeruginosa
cohabitate
with
one-another,
in
vivo.
However,
environmental
conditions
play
a
large
role
in
determining
the
dominance
of
toxic
or...
Turnip yellow mosaic virus is a monopartite, plus sense RNA virus
infecting the Cruciferae, and is a model system for the study of RNA virus
replication. A cDNA clone (pTYMC) representing an infectious RNA
genome of the European isolate of TYMV was constructed and used to assess
the importance of...
Coreplication experiments have revealed that replication of
turnip yellow mosaic virus (TYMV) RNA in turnip protoplasts is cis-preferential.
Genomes encoding mutant p141 or p66, proteins
essential for virus replication, were inefficiently rescued by a
helper genome. One model for the cis-preferential replication of
TYMV is that p66 and p141 form...
A compound series capable of reversing the antiviral effect of Ribavirin (RBV) against H1N1 was
identified from a high throughput screen designed to discover compounds capable of increasing the
fidelity of the RNA-dependent RNA polymerase (RDRP) n ribovirus quasispecies. Two lead compounds,
RIC-1 (EC50 of 78.4 nM) and RIC-2 (EC50...
Some positive-strand RNA plant viruses possess a transfer RNA-like structure (TLS) at the 3'-terminus of their genomic RNAs. The closest mimicry to tRNA is exhibited by the valylatable TLSs from tymoviruses and furo-like viruses, which are able to interact with key cellular tRNA enzymes: [CTP, ATP]:tRNA nucleotidyltransferase (CCA NTase), valyl-tRNA...
Plant viruses have been instrumental in our understanding of the biophysical properties pertaining to non-enveloped icosahedral virus particles. A substantial amount of research has been performed over five decades on Turnip yellow mosaic virus (TYMV), arguably one of the most extensively studied icosahedral plant viruses and the type-member of the...