Index Catalog // ScholarsArchive@OSU

Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and FRET/LRET Studies

Creator:: Speight, Lee C., Muthusamy, Anand K., Goldberg, Jacob M., Warner, John B., Wissner, Rebecca F., Willi, Taylor S., Woodman, Bradley F., Mehl, Ryan A., and Petersson, E. James
Abstract:: The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Resource Type:: Article

Detection techniques for biomolecules using semi-conductor nanocrystals and magnetic beads as labels

Creator:: Chatterjee, Esha
Abstract:: Continued interest in the development of miniaturized and portable analytical platforms necessitates the exploration of sensitive methods for the detection of trace analytes. Nanomaterials, on account of their unique physical and chemical properties, are not only able to overcome many limitations of traditional detection reagents but also enable the exploration...
Resource Type:: Dissertation
Full Text:: on the development of quantum dot-encapsulating liposomes as a novel fluorescent label for

WilliTaylorBiochemBiophysEfficientSynthesis.pdf

Creator:: Speight, Lee C., Muthusamy, Anand K., Goldberg, Jacob M., Warner, John B., Wissner, Rebecca F., Willi, Taylor S., Woodman, Bradley F., Mehl, Ryan A., and Petersson, E. James
Abstract:: The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:: synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and

WilliTaylorBiochemBiophysEfficientSynthesis_SupportingInformation.pdf

Creator:: Speight, Lee C., Muthusamy, Anand K., Goldberg, Jacob M., Warner, John B., Wissner, Rebecca F., Willi, Taylor S., Woodman, Bradley F., Mehl, Ryan A., and Petersson, E. James
Abstract:: The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:: Information S1 Efficient Synthesis and In Vivo Incorporation of Acridon-2- ylalanine, a

Three Sites are Better than One : Exploring Multivalency in LC8 Binding Partners

Creator:: Clark, Sarah A.
Abstract:: Hub proteins bind a large number of partners to facilitate structural changes and downstream protein interactions. LC8, a highly conserved protein homodimer, is a unique hub that regulates the activity of proteins in a wide range of cellular processes by binding to intrinsically disordered regions. With many of these systems,...
Resource Type:: Dissertation
Full Text:: less understood. In vivo studies demonstrate that ASCIZ is a transcription factor for LC856, 58 and a

Part I: Synthetic Studies toward C10-functionalized Lycopodium Alkaloids. Part II: Exploration of the Pummerer Cyclization and Its Application to the Total Syntheses of the Abietane Diterpenoids

Creator:: Li, Xin
Abstract:: This dissertation is separated into two parts according to the two major distinct research projects. In Part I, the full account of synthetic studies toward C10-functionalized lycopodium alkaloids is described. In Part II, the detailed discussion on the exploration of the Pummerer cyclization methodology and its application to the total...
Resource Type:: Dissertation
Full Text:: mainly in Asia and South America for treating various diseases.2 For example, Qian Ceng Ta (Lycopodium

Structural studies of the antioxidant defense enzymes; copper, zinc superoxide dismutase and alkyl hydroperoxide reductase flavoprotein

Creator:: Roberts, Blaine R.
Abstract:: Oxygen derived radicals are involved in many aspects of life from aging and cell signaling to disease states as diverse as heart disease, diabetes, neurodegeneration and inflammation. Therefore, understanding the function of antioxidant defense proteins and the effects of oxygen derived radicals on protein function is essential to elucidate the...
Resource Type:: Dissertation
Full Text:: fluorescent protein has been proposed as a real-time marker for tyrosine nitration in vivo. We demonstrate

Displacement-based fluorescent microRNA biosensors : design principles and analytical metrics

Creator:: Larkey, Nicholas E.
Abstract:: One post-transcriptional mechanism that regulates the progression of cancer and other diseases involves small 22-23 nucleotide sequences called microRNA (miR). Early detection of small changes in concentration of these biomarkers holds potential to diagnose diseases at their earliest stages. Use of current nucleic-acid based biosensors, like molecular beacons, for in...
Resource Type:: Dissertation
Full Text:: ...................................................................................................................66 Chapter 4. Förster resonance energy transfer impart signal-on and -off capabilities in a single

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Creator:: Blanchard, David, L.
Abstract:: L-pipecolic acid (L-PA) is the higher homolog of proline. It occurs naturally in many organisms, including primates, as an intermediate in lysine degradation. The pathway by which lysine is converted into L-pipecolic acid employs the enzyme Lpipecolate oxidase (L-PO), and appears to be tissue specific to the central nervous system...
Resource Type:: Dissertation
Full Text:: was not a substrate or inhibitor of the enzyme. The 5-hydroxy and 5,5- 2 difluoro analogs

Fluorescence studies on the bases of the nucleic acids in solution at room temperature

Creator:: Hauswirth, William Walter
Abstract:: Fluorescence of adenine, guanine, thymine, cytosine, and uracil has been detected at room temperature in neutral aqueous solution using a digital signal accumulation technique and right angle fluorescence detection from solutions with absorbances between 0. 4 and 0. 7. The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴,...
Resource Type:: Dissertation
Full Text:: thymine taken on a Turner Model 210 and a Cary Model 15. 49 1. 2 Fluorescent properties of the bases at

ScholarsArchive@OSU

Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and FRET/LRET Studies

Detection techniques for biomolecules using semi-conductor nanocrystals and magnetic beads as labels

WilliTaylorBiochemBiophysEfficientSynthesis.pdf

WilliTaylorBiochemBiophysEfficientSynthesis_SupportingInformation.pdf

Three Sites are Better than One : Exploring Multivalency in LC8 Binding Partners

Part I: Synthetic Studies toward C10-functionalized Lycopodium Alkaloids. Part II: Exploration of the Pummerer Cyclization and Its Application to the Total Syntheses of the Abietane Diterpenoids

Structural studies of the antioxidant defense enzymes; copper, zinc superoxide dismutase and alkyl hydroperoxide reductase flavoprotein

Displacement-based fluorescent microRNA biosensors : design principles and analytical metrics

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Fluorescence studies on the bases of the nucleic acids in solution at room temperature

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