A kinetics-based enzyme-linked immunosorbent assay (KELA) for detection of serum IgG antibody against bovine respiratory syncytial virus (BRSV) was developed and optimized. Optimization factors tested included brand of microELISA plate, length of incubation periods at various temperatures, type of conjugate, purified versus precipitated antigen preparations, type of dilution buffer as...
An enzyme-linked immunosorbent assay was developed to
measure specific antibody response in bovine sera to bovine
adenovirus type 3 (BA3), an etiologic agent of respiratory
disease causing economic losses annualy to the cattle
industry. Observed endpoint titers were determined using
the intersection point from optical density values of
serially diluted...
The study and utilization of Danio rerio (Zebrafish) as a model organism has increased greatly in recent decades. They are used in many areas of biological research, but one field that has used them extensively is immunology. Since zebrafish have been found to possess many orthologs to human genes, study...
To develop an assay to detect antibodies to Bacillus
thuringiensis subsp. israelensis in mallard ducks, a growth
curve was first established for the bacterium. The growth
curve indicated that the crystal delta endotoxin would be
best harvested from the rest of the cell material after 12
hours of growth. The...
Potato leafroll virus (PLRV) causes yield and quality losses in
potato. PLRV is identified by plant symptoms and serological tests
such as an enzyme-linked immunosorbent assay (ELISA). A similar
serological test, direct tissue blotting assay (DTBA), was used to
detect and monitor PLRV movement in field-inoculated Russet Burbank
plants and...