Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified
to apparent homogeneity from bacteria that were defective in uracil-DNA
glycosylase (Ung). After cloning the dug gene, recombinant Dug was
overexpressed, purified, and characterized with respect to activity, substrate
specificity, product DNA binding, and mechanism of action. Purified Dug
excised both uracil...
The Min protein system in Escherichia coli helps the cell division process by identifying the center of the cell [1, 7]. This system has been modeled succesfully computationally under standard conditions. There has been recent experimental interest in the cell division process for signiﬁcantly perturbed cell shapes. We take the...