Double-stranded cDNA was prepared from infectious hematopoietic
necrosis virus mRNA and cloned into the plasmid vector pUC8. A cDNA
clone (pG8) containing the entire coding region for the glycoprotein
(G-protein) of infectious hematopoietic necrosis virus was selected
by hybridization to a ³²P-labeled probe. The restriction map and
nucleotide sequence of...
Complementary DNA (cDNA) clones were generated from the RNA genome
of infectious hematopoietic necrosis virus (IHNV) by using random DNA
oligomers to prime first strand synthesis. These clones were mapped to
their respective locations on the genome and used to determine the
nucleotide sequence of the nucleocapsid (N) gene and...
The glycoprotein, G, of infectious hematopoietic necrosis virus, IHNV,
was shown to be the viral protein which elicits and reacts with neutralizing
antibodies. The G protein also confers protective immunity on immunized
susceptible salmonid hosts to IHNV. The other three structural proteins of
IHNV, N, Ml, and M2, do not...