Three closely related isolates belonging to the A₁ serotype of infectious pancreatic
necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of
variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were
experimentally infected with the three isolates by immersion. Cumulative mortalities over...
The glycoprotein, G, of infectious hematopoietic necrosis virus, IHNV,
was shown to be the viral protein which elicits and reacts with neutralizing
antibodies. The G protein also confers protective immunity on immunized
susceptible salmonid hosts to IHNV. The other three structural proteins of
IHNV, N, Ml, and M2, do not...
The aquaculture industry in the UK has experienced substantial growth in
the past two decades. The productivity and competitiveness of the sector to
a great extent depends on the favourable health status that the sector enjoys
relative to other major salmon producing countries in Europe. Currently the
UK is subject...
Several systems were examined for the production and delivery of recombinant vaccines for fish. C. crescentus was employed to produce a fragment of the IHNV glycoprotein. When administered by injection to 0.5 gram rainbow trout (Oncorhynchus mykiss), one of the fusion proteins (184 amino acids of the IHNV glycoprotein fused...
A characterization of the antigenic determinants (epitopes) of the glycoprotein (G) of infectious hematopoietic necrosis virus (IHNV) was made with different regions of the G gene expressed in Escherichia coli. A cDNA copy of the G gene was divided into four fragments after Taq I digestion and these fragments were...
Molecular biological approaches were used to study and interfere with the life
cycle of infectious hematopoietic necrosis virus (IHNV). These included the control
of IHN disease in rainbow trout by genetic immunization or interference in vitro by
synthesis of sense and antisense expression of the viral nucleocapsid (N) gene, and...