Flow cytometry is a highly-extensible technology for performing rapid multi-parametric particle analysis. The use of flow cytometry has proliferated with the advent of smaller, intuitive, and more affordable instruments, along with a perpetual increase in research and medical applications. However, the technology is still largely inaccessible due to cost and...
A practical and versatile instrument has been developed both to
optimize the chemistry of bio- and chemiluminescence reactions and to
measure ultra-trace quantities of associated analytes. The instrument
consists of a pneumatic flow injection system linked to a detection cell and a
signal processing and readout system. The disk-like cell...
Nuclear DNA flow cytometry was used to differentiate ploidy level and determine nuclear DNA content in Rubus. Nuclei suspensions were prepared from leaf discs of young leaves following published protocols with modifications that included: increasing the stain concentration, adding the stain after the RN ase treatment instead of adding it...
I. Frozen bovine semen samples were measured by flow cytometry
(FCM) to determine if FCM analyses could be useful for sperm quality
evaluation and prediction of bull fertility. Fluorescent dyes
utilized were acridine orange, to measure sperm nuclear chromatin
heterogeneity by quantifying susceptibility of the chromatin to
denaturation, and rhodamine...