Cytokinins are plant hormones regulating cell division and differentiation.
Most developmental events from germination, vegetative morphogenesis to seed
and fruit set are influenced by cytokinins. t,ns-Zeatin is the major natural
occurring cytokinin. Its isomer, cis-zeatin, and its derivatives are usually present in
low quantities and have traditionally been considered as...
Cytokinins can be conjugated to N- and O-glucosides. While N-glucosylation generally leads to irretrievable loss of activity, O-glucosides are thought to play an important role in cytokinin storage and homeostasis since they can be hydrolyzed to the corresponding aglycones. The first zeatin O-glycosyltransferase genes isolated were ZOG1 and ZOX1 from...
This thesis describes an investigation of the molecular basis of the substrate specificity and catalytic mechanism of a unique pseudoglycosyltransferase (PsGT) enzyme, VldE. PsGTs are a newly discovered group of glycosyltransferase (GT)-like proteins that catalyze the transfer of a pseudosugar to an acceptor molecule via a C-N bond formation. This...