Our experiments aimed to investigate responses of the marine cyanobacteria Prochlorococcus and Synechococcus to toxic arsenic species, such as arsenate, and to understand cellular mechanisms that contribute to arsenic tolerance. We used two strains of Prochlorococcus, a high-light adapted MED4 and low-light adapted MIT9313, both axenic, for cultivation experiments. For...
Even with advanced medical technology, there is still an unmet need for organs to reduce the transplant waiting list and to advance organ-related research. Cryopreservation through vitrification is one possible solution to increase organ availability. A major challenge with cryopreservation of organs is the toxicity of the cryoprotectants (CPAs) that...
Flavored milk is a growing category for dairy products; however, it is a perishable product that is subject to microbial spoilage which can lead to a negative consumer experience and lead to lost sales. Flavored milk processors communicated unique spoilage events related to their products, including color loss of strawberry...
Deep-sea chemosynthetic ecosystems remain a frontier in marine science. Essential to their role in the ocean ecosystem are endosymbiotic bacteria which support larger megafauna, including the widespread symbiont-bearing Vesicomyid clams. The diversity and characteristics of these symbionts in these clams is only just beginning to be understood with technological advances...
The safety of the chemical glyphosate, the active ingredient in the herbicide RoundUpTM, has been questioned on numerous occasions. Previous studies suggest that glyphosate-containing herbicides have deleterious effects on a variety of organisms; other studies suggest that these effects may be minor, transient, or non-existent altogether. Proper maintenance of microbial...
Corals provide a diversity of ecosystem services, are among the most biologically diverse ecosystems on Earth, and directly support ~500 million people globally; however, corals are increasingly experiencing significant threats and are undergoing severe bleaching events as the result of the warming climate. Using a two-year data set surrounding a...
Nitrification is a critical step in the global nitrogen cycle involving the biological oxidation of ammonia (NH₃) to nitrite (NO₂⁻ ) and then to nitrate (NO₃⁻). The first step in nitrification is carried out by NH₃-oxidizing bacteria (AOB) and archaea (AOA), and the second by NO₂⁻-oxidizing bacteria (NOB). In addition...
Nitrification is the process within the global Nitrogen Cycle where ammonia (NH3) is oxidized to nitrate (NO₃⁻) and can be carried out by two distinct groups of bacteria. The ammonia-oxidizing bacteria (AOB) first oxidize NH₃ to nitrite (NO₂⁻), and second, the nitrite-oxidizing bacteria (NOB) oxidize NO₂⁻ to NO₃⁻. In aerobic...
Reports of foodborne outbreaks have increased in the last decade, posing a public health risk to consumers. In an attempt to mitigate this risk, the newly established Food Safety and Modernization Act (FSMA) takes a preventive rather than reactive approach to food safety. Under FSMA, producers are required to validate...
Nitrification and denitrification are major biological processes transforming nitrogen (N) in soils to plant available N, highly leachable nitrate (NO₃⁻) and gaseous N oxides. Although many studies in the past have studied N cycling communities, the effect of increased N inputs on ammonia-oxidizer and denitrifier population dynamics is still under...