The two segments of double-stranded RNA (dsRNA) from
infectious pancreatic necrosis virus (IPNV) were cloned
into plasmid vector pUC8. Two distinct sets of overlapping
clones were identified by restriction enzyme and Southern
blot analysis. Each of these sets was shown, by Northern
blot analysis, to be exclusively related to either...
Complementary DNA (cDNA) clones were generated from the RNA genome
of infectious hematopoietic necrosis virus (IHNV) by using random DNA
oligomers to prime first strand synthesis. These clones were mapped to
their respective locations on the genome and used to determine the
nucleotide sequence of the nucleocapsid (N) gene and...
The glycoprotein, G, of infectious hematopoietic necrosis virus, IHNV,
was shown to be the viral protein which elicits and reacts with neutralizing
antibodies. The G protein also confers protective immunity on immunized
susceptible salmonid hosts to IHNV. The other three structural proteins of
IHNV, N, Ml, and M2, do not...
A rapid virus detection method was developed for Infectious Hemotopoietic Necrosis Virus (IHNV). The virus detection method could be applied to Infectious Pancreatic Necrosis Virus (IPNV) under certain conditions. It was possible to detect IHNV to a lower limit of 1.7 X 10⁴ Tissue Culture Infectious Dose for 50% (TCID₅₀)...
DNA polymerase gamma has been purified over 67,500-fold from human trophoblast tissue. Purification was achieved through successive chromatographies on phosphocellulose, DEAE-cellulose, DNA-cellulose, and high pressure gel exclusion through Fractogel TSK HW55. This preparation yielded a specific activity of 81 units/mg of protein using the preferred template-primer, poly (A)-oligo (dT)₁₂₋₁₃. Analysis...
The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
Double-stranded cDNA was prepared from infectious hematopoietic
necrosis virus mRNA and cloned into the plasmid vector pUC8. A cDNA
clone (pG8) containing the entire coding region for the glycoprotein
(G-protein) of infectious hematopoietic necrosis virus was selected
by hybridization to a ³²P-labeled probe. The restriction map and
nucleotide sequence of...
The immune systems of various teleost fish have been studied in some detail for
the past several decades. One aspect of fish immunity, that of endogenously produced
modulating factors, has recently received a great deal of attention. Understanding the
functions and roles of endogenous factors that regulate fish immunity is...
A characterization of the antigenic determinants (epitopes) of the glycoprotein (G) of infectious hematopoietic necrosis virus (IHNV) was made with different regions of the G gene expressed in Escherichia coli. A cDNA copy of the G gene was divided into four fragments after Taq I digestion and these fragments were...