The influence of substrate and enzyme concentration on the rate
of saccharification of two defined, insoluble, cellulose substrates,
Avicel and Solka-Floc, by the cellulase enzyme system of Trichoderma
viride has been evaluated. Assays utilized enzyme concentrations
ranging from 0.014 to 0.056 filter paper unit per mL and substrate
concentrations up...
A novel type of model substrates, i.e. immobilized p-aminophenyl-β-D-cellooligosaccharides,
was developed and used in the study of exocellulases. The
two major cellobiohydrolases from Trichoderma reesei, CBH I and CBH II were
used as representative enzymes. p-Aminophenyl derivatives of cellobiose (PAPG₂),
cellotriose (PAPG₃), and cellotetraose (PAPG₄) were synthesized from the reaction...
The objective of this research was to investigate the influence of physicochemical
properties of lignocellulosic biomass on the rate and extent of cellulose conversion, i.e.
the "hydrolyzability" of the substrate.
The initial two parts of this thesis investigated the interactions between various
components of pretreated biomass. The relevance of cellulase...
The traditional filter paper assay for saccharifying cellulase
originally described by M. Mandels et al (1976) has been modified to
make possible low activity determinations of Trichoderma
cellulases. The enzymatic activity appears to decline during a
prolonged incubation period if no precautions have been taken. By
means of adding bovine...
Increasingly, forest management and conservation plans require spatially explicit information within a management
or conservation unit. Forest biomass and potential productivity are critical variables for forest planning and
assessment in the Pacific Northwest. Their values are often estimated from ground-measured sample data. For
unsampled locations, forest analysts and planners lack...
The research described in this thesis was aimed at understanding how particle size, porosity, and enzyme accessible surface area influence the rate of saccharification of microcrystalline cellulose. Microcrystalline cellulose (MCC) is a commonly used substrate for the study of cellulolytic enzymes. MCC preparations of different particle size are commercially available....
The effect of dietary glycosylated vitamin B-6 on the bioavailability
of vitamin B-6 was determined in 4 women. A 44-d metabolic-balance diet
study was divided into a preliminary 8-d adjustment period followed by
two 18-d experimental periods. The subjects were divided into two groups
in a crossover design to compare...
Two related techniques, based upon ion-exchange solid phase extraction, have been
developed for the determination of arsenic speciation. The inorganic arsenic species
arsenite (As(III)) and arsenate (As(V)) are separated by anion-exchange and detected with
graphite furnace atomic absorption spectrophotometry (GFAAS) with a nickel matrix
modifier. The first separation technique, which...