A sensitive assay for the analysis of contaminating segments
in pseudorecombinants constructed from a seed-transmissible (CVM-Pg)
and a non-seed-transmissible (CMV-Le) strain of cucumber mosaic
virus was developed using partial RNase T₁ digestion. Isolated
genomic RNA segments produced unique digestion patterns when 3'-
end labeled, treated with RNase T₁, and subjected...
Polyclonal antisera were raised against isolates of bean
common mosaic virus (BCMV) and bean common mosaic necrosis
virus (BCMNV) using conventional serological methods.
Infected tissues containing, respectively, 22 recognized BCMV
and BCMNV isolates were tested against the two antisera by
antigen-coated plate (ACP) ELISA and double antibody sandwich
(DAS) ELISA....
A survey of major bean (Phaseolus vulgaris) breeding programs and
seed producing areas in Idaho, Washington, and Oregon was conducted to
determine the occurrence of cucumber mosaic virus (CMV), recently reported
to be seedborne in beans. Some 259 plants, sampled from breeding
lines of diverse germplasm, were found by enzyme-linked...