A mathematical model of Limiting Dilution Analysis for two limiting
parameters is presented and investigated. Limiting Dilution Analysis is a microbiological
cell assay developed for immunological application. In the given
case we deal with the interaction between B lymphocytes, macrophage derived
factor and T-independent antigens. The state of the art...
A casein agar diffusion method was developed to detect and quantify pertinent
levels of proteinases produced in raw milk supplies by heat resistant Bacillus
sporeformers. In order to optimize the required heat treatment conditions of raw milk
samples, trials that involved a combination of different temperatures and times were
evaluated....
The traditional filter paper assay for saccharifying cellulase
originally described by M. Mandels et al (1976) has been modified to
make possible low activity determinations of Trichoderma
cellulases. The enzymatic activity appears to decline during a
prolonged incubation period if no precautions have been taken. By
means of adding bovine...