Sporulation in the yeast Saccharomyces cerevisiae involves
the process of meiosis which is accompanied by ascospore formation.
Using intact cells or spores, preliminary biochemical evidence is
given to identify ascospore wall components not found in vegetative
cell walls. Cell surface components are solubilized by treatment
with 8 M urea and...
Six plant pathogenic isolates of Pseudomonas
syringae pv. glycinea, the causal agent of bacterial
blight of Glycine max (L.) Merrill, and one isolate of
P. syringae pv. phaseolicola, the causal agent of halo
blight of Phaseolus vulgaris L., have been examined for
the presence of circular duplex deoxyribonucleic acid
A pathogenicity locus of Pseudomonas syringae pv.
syringae identified by Tn5 mutagenesis was investigated.
The mutant strain PS9024 is attenuated for disease
expression in its host, Phaseolus vulgaris, but produces
the hypersensitive reaction (HR) in the nonhost, tobacco
(Nicotina tabacum). A cosmid clone carrying 16 kilobases
(kb) of contiguous genomic...
Inbreeding of the avirulent physiologic race 8 strains of Ustilago hordei was
purported to have increased its pathogenicity in a stepwise manner that led to a highly
pathogenic race, designated race 14. The analysis of electrophoretic karyotypes and
restriction fragment length polymorphism profiles detected with a telomere-specific probe
1S801 is a transposable element isolated from Pseudomonas syringae pathovar
(pv.) phaseolicola, the causal agent of halo blight of bean. Fragments of the element are
present in multiple copies on an indigenous plasmid, pMMC7105, of strain LR781, and
have been implicated as sites of homologous recombination leading to imprecise
Genetic studies of Tilletia spp. have been hampered by the lack
of genetic markers. The work discussed here is a first report of the
isolation of genetically marked strains of J. caries and their use in
wheat plant infection studies. Procedures for the isolation of auxotrophic and drug-resistant mutants in...
Five strains of the bean pathogen Pseudomonas syringae pv.
phaseolicola were analyzed for plasmid number, size, and relatedness.
Each strain contained one or two indigenous plasmids which ranged in
size from 6.8 to 151 kbp. BamHI and EcoRI restriction patterns revealed that total plasmid DNA from each strain had a...
A strain of the phytopathogenic bacterium Pseudomonas syringae
pv. phaseolicola, a pathogen of common bean Phaseolus vulgaris,
harbors a 150 kilobase pair (kb) plasmid pMC7105. In one strain,
LR719, pMC7105 is stably integrated in the bacterial chromosome.
Imprecise excision of the pMC7105 replicon has been shown to result