miRNA-guided cleavage initiates entry of primary transcripts into the trans-acting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6 (RDR6), DICER-LIKE 4 (DCL4), and SUPPRESSOR OF GENE SILENCING3 (SGS3). Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173- guided initiation cleavage on the 5’ side of the siRNA-generating...
Transacting siRNA (tasiRNA) biogenesis in Arabidopsis is initiated
by microRNA (miRNA) –guided cleavage of primary transcripts. In
the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)–
miR390 complexes interact with primary transcripts at two sites,
resulting in recruitment of RNA-DEPENDENT RNA POLYMERASE6
for dsRNA biosynthesis. An extensive screen for Arabidopsis mutants...
In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
Endogenous small interfering RNAs (siRNAs) are a class of naturally occuring regulatory RNAs found in fungi, plants, and animals. Some endogenous siRNAs are required to silence transposons or function in chromosome segregation; however, the specific roles of most endogenous siRNAs are unclear. The helicase gene eri-6/7 was identified in the...
The improvement of coupled tendon transfer surgeries through the insertion of an adaptive engineering mechanism was explored using biomechanical simulations performed in OpenSim. Tendon transfer surgeries are commonly performed on the upper extremity to restore hand function. A conventional 4-tailed tendon transfer procedure model and a modified 4-tailed procedure model...
Wildfire management policy over the past century, which attempts to exclude fire from fire-adapted ecosystems, has led to a build-up of fuels across the western United States. As a result, current wildfires contain larger areas of high severity, high intensity burns than seen prior to the policy implementation. There are...
This work examined the transformation of the concept of biodiversity into natural resource policies of the U.S. Agency for International Development and the World Bank through 1988. The study identified several groups as playing key roles in the transformation process. These were nongovernmental environmental organizations, the scientific community, and the...
This research project dives deep into the life cycle and multiple disruptions of the vacation rental management industry as whole, and specifically in Sunriver, Oregon. It uses primary research through interviews of dozens of professionals in the industry along with secondary research to outline the markets life cycle and disruption....
The use of advanced concepts can make the nuclear fuel
cycle more efficient by allowing pins to be operated at
high powers and to higher burnups. Among the more
promising concepts in advanced fuels is sphere pac fuel.
In a first attempt to bring together models describing
individual phenomena, the...
The thesis presented here is a quantitative study of vacant
urban land in Corvallis, Oregon. In order to present this study the
writer first studied the available literature and found that little had
been published dealing with causal factors of vacant urban land.
Rather, it was found that several writers...