Cell division in a heat synchronized Tetrahymena pyriformis
(GL-I) cell can be delayed if the cell is exposed to X-irradiation (300
kVp, 20 mA, HVL = 0. 9 mm Cu, 25 R/ sec) prior to a critical time
after the end of the synchronizing treatment (EST). At the critical
time,...
During karyokinesis in the ciliate, Tetrahymena pyriformis, strain GL-I, an extranuclear chromatin body (ECB) is frequently formed as an excluded mass between the two dividing daughter macronuclei. The formation and fate of ECB's were followed by means of Feu lgen, toluidine blue 0, and euchrysine staining and autoradiography (³H-thymidine). The...
The primary objective of this study was to develop methods for
the selection of temperature sensitive division (TSD) mutants of
Chlamydomonas reinhardtii. Such methods were developed and four
temperature sensitive (TS) mutants were isolated and partially
characterized.
Wild type (WT) cells of the plus mating type were synchronized
by a...
Hydroxyurea (10mM) blocks the exponential growth of Tetrahymena
pyriformis (GL-I) populations by arresting progress through the cell
cycle once the cells enter S-phase. Autoradiographic analysis reveals
that HU reduces the uptake of tritiated thymidine (³H-TdR) into
macronuclear DNA in S-phase cells to about 1/5 of that in untreated
S-phase cells....