The first goal of this study was to understand the role of calpains in skeletal muscle
protein degradation in cultured cells. We have developed a genetic approach to inhibit
endogenous calpain activity through over-expressing dominant negative m-calpain (DN),
antisense m-calpain (AS) and calpastatin inhibitory domain (CID). We observed that,
under...
OmniGen-AF® (OG; Prince Agri Products, Inc., Quincy, IL) is a feed additive containing brewers dehydrated yeast and B-vitamins that supports immune function in ruminant livestock. Targeted profiling of immune-associated genes in whole blood is an effective platform for identification of a) novel molecular pathways and b) immune response markers to...
Glucocorticoids are growth-inhibiting steroids. They have been reported to reduce
muscle growth by reducing protein synthesis. However, their actions on muscle protein
degradation remain equivocal. Glucocorticoids have been reported to transiently increase
muscle protein degradation, to not affect this process and to reduce muscle protein
degradation. Reasons for these conflicting...
Objectives of this study were to examine effects of age, castration and gestation on
actvities of calpains and calpastatin in skeletal muscle. Also, the regulation of calpains
and calpastatin at the molecular level was investigated. Two experiments were designed.
Experiment 1 was designed to evaluate effects of age and castration...
Skeletal muscle proteins are the largest amino acid pool in animal body and are continuously degraded and resynthesized. Dozens of factors have been shown to influence the balance between synthesis and degradation and thereby regulate muscle growth and function. It is now know that one of the major regulatory bases...