The 105,000 x g supernatant fluid (S[subscript HS]) from extracts of pea seedlings
(Pisum sativum, var. Alaska) etiolated for 60 hours yielded a
very potent adenosine triphosphatase activity. This activity was designated
as an apyrase when the reaction stoichiometry indicated that
adenosine triphosphate (ATP) was hydrolyzed to adenosine monophosphate
(AMP)...
Effects of phorbol myristate acetate (PMA), dibutyryl cyclic
AMP (dbcAMP), 6-dimethylaminopurine (6-DMAP), okadaic acid
(OA), cycloheximide (CHX), actinomycin D (AcD) and tunicamycin
(TuM) on plasminogen activator (PA) activity and maturation rate
in bovine and porcine oocyte-cumulus cell complexes (BOCC and
POCC, respectively) in vitro were determined. Plasminogen
activator activity was...
Our objectives were to determine 1) the concentration of prostaglandin
F2α (PGF2α) in whole and extended bovine semen, 2) if concentrations of
prostaglandins in extended bovine semen are correlated to fertility, and 3) if PGF2α
administered at the time of artificial insemination would improve conception rate.
Concentration of PGF2α tended...
Acetylimidazole was used to acetylate the tyrosyl residues
of insulin. The kinetics of acetylation were studied by termination of
the reaction at several time intervals, isolation of acetylated insulin
and determination of 0-acetyltyrosyl groups by deacetylation with
hydroxylamine. The deacetylation was also studied kinetically by
means of the absorbancy change...
Polypeptidyl derivatives of bovine pancreatic ribonuclease
(RNase) containing two or three tyrosine chains
with an average of two to three residues per chain have
been prepared. The effects of various perturbants such as
neutral salts, ethanol, urea and guanidine hydrochloride
on the thermal transition of the derivatives have been
studied....
Drosophila suzukii was a known pest of berries, grapes, and stone fruits in East Asia for almost a century. Yet in recent years it has successfully migrated throughout the United States, Mexico, and Europe due to globalized fruit trade. The invasive success of D. suzukii is causing unrest for fruit...
Steroid organoboranes have been found to be useful synthetic
intermediates. Attempted pyrolyses, based on simple trialkylborane
cyclizations, have not yielded C-19 or C-18 functionalized steroids,
but have produced isomerized systems not otherwise available.
Thus, hydroboration of cholesterol followed by pyrolysis at
215° gives 31% of 2-cholestene-6α-ol and 49% of a...