Breast cancer is a leading cause of cancer deaths among women, with an estimated 12.4% of American women diagnosed with this tumor type. Evidence suggests that exogenous disruptors can exert epigenetic changes leading to aberrant gene expression, shifting the cellular balance toward oncogenesis. While chemical pollutant exposure is well-studied, light...
Canine transitional cell carcinoma (TCC) has been shown to have a substantial inflammatory component. It exhibits genotypic and morphologic elements that resemble its human counterpart. Cyclooxygenases (COX) are key enzymes in the synthesis of proinflammatory molecules, such as prostaglandins. Excess prostaglandin production through cyclooxygenases may promote oncogenesis and progression of...
The effect of vitamin B-6 (VB6) deficiency in mice on host
susceptibility to primary and secondary Moloney-sarcoma virus
(MSV)-induced tumor growth, cytotoxic activities of T cells,
antibodies and natural killer (NK) cells, and phagocytosis by
macrophages was examined. Five- to six-week old female C57B1/6 mice
were fed 20% casein diets...
Research in Dolan lab focuses primarily on the adaptive immune system and ways it can be better utilized to eliminate infected or transformed cells. Research focuses on the major histocompatibility (MHC) class I antigen presentation pathway, which plays an important role in alerting the immune system of the presence of...
The isothiocyanate sulforaphane is a promising molecule for development as a therapeutic agent for patients with metastatic
prostate cancer. Sulforaphane induces apoptosis in advanced prostate cancer cells, slows disease progression in vivo and is well
tolerated at pharmacological doses. However, the underlying mechanism(s) responsible for cancer suppression remain to be...
Full Text:
sulforaphane-induced apoptotic signaling in PC3 prostate cancer cells.
Oncogenesis, 3, e131. doi:10.1038
Dysregulation of Sonic hedgehog (Shh) signaling has been implicated in glioma pathogenesis. Yet, the role of this pathway
in gliomagenesis remains controversial because of the lack of relevant animal models. Using the cytokeratin 5 promoter, we
ectopically expressed a constitutively active zebrafish Smoothened (Smoa1) in neural progenitor cells and analyzed...
A retrovirus homologue gene of cellular cyclin D₁, walleye dermal sarcoma virus rv-cyclin
gene (orf A or rv-cyclin), was expressed in the livers of zebrafish under the control
of liver fatty-acid binding protein (lfabp) promoter. To prevent possible fatality caused
by over-expression of the oncogene, the GAL4/UAS system was used...
Transcription factors with multiple post-translational modifications (PTMs) are not uncommon, but comprehensive information on site-specific dynamics and interdependence is comparatively rare. Assessing dynamic changes in the extent of PTMs has the potential to link multiple sites both to each other and to biological effects observable on the same time scale....
Type I collagen fibers, the connective tissue that makes up the human breast, can be easily manipulated by cells or other outside factors. In previous studies, the alignment of collagen has been associated with the beginning of metastatic cancer, possibly influencing cancerous cells to migrate from the tumor. In this...
Many humans and canines alike suffer from osteosarcoma, which is often metastatic, resistant to chemotherapy, and frequently fatal. The mechanisms through which osteosarcoma cells acquire resistance to chemotherapy are not entirely understood, and the role of exosomes as intercellular mediators of this resistance in osteosarcoma is unknown. Using established canine...