Na⁺/H⁺ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na⁺/H⁺ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na⁺/H⁺ antiporters, NhaA and NhaB, are completely attenuated in...
We found that a strains of Yersinia pestis (KIM5) which lacked the nhaA gene was fully attenuated in a plague model. This gene produces a protein of the sodium-proton antiporter family which expel sodium ions from the bacterial cytoplasm in exchange for hydrogen ions, or protons, from the surrounding environment....
Full Text:
Winogrodzki, Erin Lind1, GregoryV. Plano3, Joseph B. Hinnebusch4,
Pavel Dibrov5, Claudia C. Häse1,2*
1
We found that a strains of Yersinia pestis (KIM5) which lacked the nhaA gene was fully attenuated in a plague model. This gene produces a protein of the sodium-proton antiporter family which expel sodium ions from the bacterial cytoplasm in exchange for hydrogen ions, or protons, from the surrounding environment....
Full Text:
, Erin Lind1, GregoryV. Plano3, Joseph B. Hinnebusch4,
Pavel Dibrov5, Claudia C. Häse1,2*
1 Department
We found that a strains of Yersinia pestis (KIM5) which lacked the nhaA gene was fully attenuated in a plague model. This gene produces a protein of the sodium-proton antiporter family which expel sodium ions from the bacterial cytoplasm in exchange for hydrogen ions, or protons, from the surrounding environment....
The Willamette River flows north between the Cascade Mountains and Coast Range of western Oregon within a 29,728-km² basin. The fish community in the geologically young basin consists of 36 native species, but introductions of non-native fishes have added 33 additional species for a total of 69 fish species. During...
In the Pacific Northwest, regulatory agencies have recently
implemented management strategies for restoration and maintenance of wood
recruitment to streams over time. This allochthonous organic material is a
critical component in the geomorphic and biological structure of forested
streams. Mathematical models are commonly used to evaluate long-term
wood dynamics in...
Conceptually, the dynamics of wood in streams can be viewed in terms of input and in-channel processes. Input processes are associated with both the riparian (tree fall, bank cutting, windthrow) and upslope forests (mass failures). In-channel processes include log breakage, movement, and decomposition. A mechanistic view of these processes is...
Land use alters the physical and biological structure of stream ecosystems and potentially alters their capacity to process nitrogen (N), an essential nutrient that has nearly doubled in abundance on the biosphere
during the past century from human activities. In this dissertation, I quantified uptake and transformation of nitrate (NO₃⁻)...
Understanding food webs is fundamental in conserving endangered species and maintaining healthy ecosystem function, particularly in desert spring systems. We identified dominant energy sources in the Muddy River Warm Springs area, Clark County, NV using carbon and nitrogen natural abundance stable isotope analyses. We examined isotopic signatures of specific macroinvertebrate...
The morphology and ecological importance of habitats associated with the margin of third-order stream channels in the Oregon Cascades were studied in streams in three different riparian types: 450-year-old conifers, 40-year-old alder, and an open, herbaceous-dominated clearcut. Margins, backwaters, and side channels in streams create important habitat for fry of...