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  • Influence of polyphosphates on the emulsifying capacity of milk and meat proteins

    Creator:
    Cummins, Armando
    Abstract:
    The influence of polyphosphates upon the emulsification of enzymatic hydrolyzates of casein and lactalbumin and upon salt-soluble meat proteins was determined by a model system in which oil-in-water emulsions were formed. Sodium acid pyrophosphate, sodium tripolyphosphate and sodium hexametaphosphate were mixed together in a weight ratio of 4:2:4, respectively, to...
    Resource Type:
    Masters Thesis
    Full Text:
    solution, was determined for the above proteins with or without polyphosphates at varying protein

  • Circular dichroism spectroscopy and the conformational analysis of globular proteins in the native and denatured state

    Creator:
    Compton, Larry A.
    Abstract:
    A simple matrix method for the analysis of protein circular dichroism (CD) spectra is presented and used to compute the secondary structure of globular proteins in their native and denatured states. The method uses singular value decomposition and generalized inverse algorithms, and a basis set of CD spectra for proteins...
    Resource Type:
    Dissertation
    Full Text:
    method for the analysis of protein circular dichroism (CD) spectra is presented and used to compute the

  • Functional analysis of novel β-catenin mutants

    Creator:
    Al-Fageeh, Mohamed B.
    Abstract:
    β-catenin is a multi functional protein that is involved in cell-cell adhesion and cell signaling. In non-stimulated cells, β-catenin is tightly down-regulated by GSK-3β-dependent phosphorylation at Ser and Thr residues, followed by rapid ubiquitination and proteasomal degradation. It is well established that mutations within the regulatory GSK-3β region lead to...
    Resource Type:
    Masters Thesis
    Full Text:
    functional protein that is involved in cell-cell adhesion and cell signaling. In non-stimulated cells

  • Proteomic approach to the analysis of DNA-binding proteins using mass spectrometry

    Creator:
    Stapels, Martha Degen
    Abstract:
    In proteomic studies, separate experimental protocols have been necessary to identify proteins, determine their function, and predict their three-dimensional structure. In this study, a function-based separation of proteins was conceived to fractionate proteins prior to enzymatic digestion. In the initial demonstration of this technique, a DNA substrate was used to...
    Resource Type:
    Dissertation
    Full Text:
    lysate in order to identify protein function and to simplify the complex mixture of proteins. A total of

  • Mass spectrometric analysis of proteins and peptides : elucidation of the folding pathways of recombinant human macrophage colony stimulating factor beta

    Creator:
    Zhang, Yuan Heidi
    Abstract:
    Recombinant human macrophage colony stimulating factor beta (rhm-CSFβ) is a glycoprotein that stimulates the proliferation, differentiation and survival of cells belonging to the monocyte-macrophage lineage. It contains nine inter-subunit and intra-subunit disulfide bonds and represents an excellent model system for studying disulfide bond formation during protein folding because the assembly...
    Resource Type:
    Dissertation
    Full Text:
    bonds and represents an excellent model system for studying disulfide bond formation during protein

  • Comparison of carbon nanotube and graphene field-effect transistor biosensors

    Creator:
    Saltzgaber, Grant William
    Abstract:
    Detection of biomolecules is important for the diagnosis and treatment of diseases. Low concentration detection, specific biomolecule detection, and point-of-care use are appealing characteristics for biosensors because of the possibility of early detection and quick results of specific biomolecules. Furthermore, inexpensive biosensors are appealing so that they are accessible to...
    Resource Type:
    Masters Thesis
    Full Text:
    were conducted; one using electrostatic attraction to bind protein to the active area of the

  • Proteomic analyses of marine bacteria

    Creator:
    Sowell, Sarah M.
    Abstract:
    Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
    Resource Type:
    Dissertation
    Full Text:
    . Global and soluble/insoluble protein preparation with bead beating…………… 79 3.3.4.2. Global

  • Analysis of the maize TOUSLED-LIKE KINASE gene family

    Creator:
    Yoon, Elizabeth S.
    Abstract:
    Protein kinases are an abundant class of enzymes which play important roles in numerous signal transduction systems. Arabidopsis TOUSLED kinase is a serine/threonine kinase which is essential for cell-cell communication within the shoot meristem. TOUSLED is encoded by a single gene in Arabidopsis. Recessive mutants in this gene show mild...
    Resource Type:
    Masters Thesis
    Full Text:
    . Abstract approved: Carol J. Rivin Protein kinases are an abundant class of enzymes which play important

  • Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

    Creator:
    Zhong, Lingxiu
    Resource Type:
    Dissertation
    Full Text:
    for the environmental effects on protein folding. We found that somatostatin, which has both a-helix

  • Genetic variation of chlamydial Inc proteins

    Creator:
    Viratyosin, Wasna
    Abstract:
    Genomic analysis is a new approach for the characterization and investigation of novel genes, gene clusters, the function of uncharacterized proteins, and genetic diversity in microorganisms. These approaches are important for the study of chlamydiae, a system in which several genomes have been sequenced but in which techniques for genetic...
    Resource Type:
    Dissertation
    Full Text:
    . Fluorescence microscopy showed that IncA, a chiamydial protein localized to the inclusion membrane, was