The ability of ovine endometrium incubated in vitro with
estradiol -17(3 to synthesize a specific induced protein (IP) was
studied. Endometrium from cycling, pregnant and ovariectomized
ewes was used.
Twelve ewes were allotted in equal numbers to be sacrificed on
days 0 (estrus), 3, 6, or 10 of the estrous...
Ribosomes are ribonucleoprotein particles that are essential for
the process of protein synthesis in all living systems. Escherichia
coli, eubacterial organisms, have ribosomes which display a 70S
sedimentation coefficient. They are formed by the association of two
ribonucleoprotein subunits which sediment at 50S and 30S. The 30S
subunit is a...
The ribosome is a central component of the protein
synthetic apparatus. It is a macromolecular complex of
protein and RNA. Although much progress has been made in
understanding the functional role of the proteins in this
particle, little is known of the functional role which
the RNA plays. Naturally occurring...
Vaccinia virus (VV) is a large DNA virus belonging to the Orthopoxvirus
family. The viral replicative life cycle takes place solely within the cytoplasm
of a mammalian host cell. The VV genome contains 196 open reading frames
which are expressed in a highly regulated and temporal fashion in order to...
Escherichia coli 16S-like ribosomal RNAs from
eubacterial, archaebacterial and eukaryotic organisms have
several universally conserved sequence zones which are
proposed to be single-stranded. A clear function for all
of these regions has not yet been defined. To evaluate the
roles of these sequence zones in protein synthesis,
knowledge of their...
In recent years, the general public has recognized the dangers of a high fat diet and
are demanding meat with lower fat content. This demand has stimulated research in the
growth and regulation of adipocytes. However, despite much effort, no adipocyte-specific
plasma membrane markers from any species are available as...
The ability to faithfully replicate DNA is dependent upon the maintenance
and regulation of its precursors, the deoxyribonucleoside triphosphates.
Enzymes encoded by the bacteriophage T4 have been widely used as models
of biochemical processes. A body of evidence supports the concept that the
bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Vaccinia virus infected monkey kidney cells had been previously shown
to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA
from mutant virus resistant to hydroxyurea were digested with restriction
endonucleases and were shown to have substoichiometric amounts of the Hind
III F fragment. Additional information from Southern blotting experiments...
With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...