Under defined cultural conditions, zygotes of the brown alga
Fucus distichus L. Powell divide synchronously to form two-celled
embryos at approximately 24 hours after fertilization. These two
cells differ from one another in gross morphology, ultrastructure,
cytochemistry, and developmental fate. Cytochemical staining and
autoradiography indicated that a sulfated polysaccharide was...
Under defined cultural conditions, large populations of zygotes
of the brown algae Fucus vesiculosus L. and F. distichus L. Powell
synchronously form a cell wall shortly after fertilization. Intact cell
walls, free of cytoplasm and maintaining shape properties of early
stages of embryo development, have been isolated from these zygotes....
Fucus distichus L. Powell zygotes were used to investigate
cell wall synthesis and development and to construct a model of
cell wall structure.
Alginate synthesis was investigated. Zygotes were cultured
in seawater containing ¹⁴C-bicarbonate. Alginate represented
12%, 37%, and 1.7% of the label recovered from whole zygotes,
wall, and cytoplasm,...
Germination of immature (Stage III) and mature (Stage V) wheat (Triticum aestivum L. var. Chinese Spring and Yamhill) embryos is prevented by the inclusion of 10⁻⁴ M abscisic acid (ABA) in culture medium. In addition, Stage III embryos develop into mature embryos in culture medium containing ABA. In the absence...