A new photosystem-II mutant of the green alga Scenedesmus
obliquus D₃, strain PS-28, has been shown to lack a-tocopherol
(vitamin E). The photosynthetic activity of dark grown samples of
PS-28 is about 20% of the wild-type control. Culturing the mutant
at low light intentisites (10⁴ ergs/sec-cm ²) stimulates photosynthetic
activity...
E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...