Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
Motivation: It can take considerable amounts of time and effort to use existing computational tools to analyze a small RNA sequence for the purpose of determining if it is characteristic of microRNA. It is similarly tedious to search for homologs to known microRNA sequences in the rapidly growing collection of...
Nitrogen is one of the major nutrients limiting microbial productivity in the ocean, and as a result, most marine microorganisms
have evolved systems for responding to nitrogen stress. The highly abundant alphaproteobacterium “Candidatus
Pelagibacter ubique,” a cultured member of the order Pelagibacterales (SAR11), lacks the canonical GlnB, GlnD, GlnK, and...
Full Text:
PII-Independent Response to Nitrogen
Limitation in a Free-Living Alphaproteobacterium
DanielP. Smith
Nitrogen is one of the major nutrients limiting microbial productivity in the ocean, and as a result, most marine microorganisms
have evolved systems for responding to nitrogen stress. The highly abundant alphaproteobacterium “Candidatus
Pelagibacter ubique,” a cultured member of the order Pelagibacterales (SAR11), lacks the canonical GlnB, GlnD, GlnK, and...
Nitrogen is one of the major nutrients limiting microbial productivity in the ocean, and as a result, most marine microorganisms
have evolved systems for responding to nitrogen stress. The highly abundant alphaproteobacterium “Candidatus
Pelagibacter ubique,” a cultured member of the order Pelagibacterales (SAR11), lacks the canonical GlnB, GlnD, GlnK, and...
Two experiments were conducted utilizing crossbred gilts to evaluate follicle characteristics at puberty and to determine the effect of exogenous estradio1-17β on embryonic mortality in the mature cycling gilt. Experiment 1 was conducted to compare preovulatory follicle numbers and sizes and follicular fluid concentrations of progesterone, estradio1-17β, plasminogen activator and...
The status of vitamin B-6 (B6) nutriture of nine
persons (4F;5M) with insulin dependent diabetes mellitus
(IDDM), nine persons (5F;4M) with non-insulin dependent
diabetes mellitus (NIDDM), and 18 control individuals
(9F;9M) was evaluated, using biochemical and dietary
indicators of B6 status. The biochemical indices employed
were plasma concentration of pyridoxal...
Vessel traffic places the sensitive environmental, natural, and cultural resources of Washington’s outer coast at risk of oil or hazardous materials spills. In 1995, International Maritime Organization established the Area to be Avoided off the Washington Coast (ATBA) to reduce this risk. Annual estimates of vessel compliance with the ATBA...
Aphids are a widespread family of plant pests, whose abilities to suppress shoot
and root growth are well documented for many terrestrial plants. Only a few studies have
been conducted on conifer aphids of the genus Cinara. Cinara pseudotsugae are known
to attack Douglas-fir seedlings, an important crop in the...
Water is an absolute necessity for life as we know it. It provides a useful medium in which chemical reactions take place that allowed for the development of single cellular organisms. When combined with the evolutionary accident that was pho- tosynthesis, water became not only a useful medium chemical reactions,...
