The release of transgenes into the environment by transgenic plant species is a significant ecological concern, and consequently a containment method for these genetically modified organisms is highly sought after. For poplar trees, an option for containment is to produce trees that do not flower and therefore cannot spread genetic...
A method for Agrobacterium-mediated transformation of hybrid
poplar (Populus alba x P. grandidentata) suspension cultures and
regeneration of transformed plants is described. The best protocol
was one in which suspension cultures were inoculated with
Agrobacterium tumefaciens to a density of 10⁷ cfu's/ml, cocultivated
for 48 hours, plated to cellulose acetate...
Stable expression of transgenes is required for commercial uses of genetically engineered trees. To better understand the stability of transgene expression under field conditions, we studied transgene expression and RNA interference (RNAi)-induced transgene suppression in 2,480 transgenic poplars (460 transgene insertion events) over three years. Stability of expression was assessed...