In the determination of binding heterogeneity and rotational
diffusion relaxation times, fluorescence decay curves often take the
form of sums of exponential decay times. An instrument is described
which includes features permitting the collection of high precision
time decay data, which may successfully be resolved into component
decays.
The instrument...
This work represents the first study of the conformational changes in histone H1 subfractions as well as the interactions of these subfractions with two nonhistone chromosomal (NHC) proteins. Calf thymus Hi was fractionated by the method of Kincade and Cole (1966a) using a very shallow guanidinium chloride gradient. A possible...
In this work, the four yeast inner histones were prepared by the
use of consecutive slab gel fractionation. The use of this technique
allowed the first complete fractionation and rigorous identification of
the four yeast inner histones, and made possible a study of their
complexing behavior.
Variants and adjuncts of...
I examined, in three separate studies, the active chromatin
structure of the yeast, Saccharomyces cerevisiae.
Yeast contains four proteins having amino acid compositions
typical of the high mobility group (HMG) proteins. Three of these
are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted
by 0.25...
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...
The interaction of polylysine with DNA may be divided into two
separate phenomena: the primary interaction involved in formation of
a well-defined complex, and secondary interactions leading to aggregation
of these complexes. Most studies on the interaction of polylysine
with DNA have used techniques which measure properties of
the aggregated...
The mechanism of continuously chlorinated silver-silver
chloride electrode performance was studied by using a wiped, rotating,
partially submerged, cylindrical silver cathode. The cell
performance using a zinc anode was measured, and the rate of
chlorination of silver was calculated for several water saturated
chlorine atmospheres.
At 75°F, with a chlorine...
I have investigated nucleoprotein organization in
chromatin of the yeast, Saccharomyces cerevisiae. My
approach has been to prepare insoluble, minimally per
turbed chromatin from a mitochondrial DNA-free strain of
yeast, using a number of new techniques, and then digest
this substrate with micrococcal nuclease. The soluble
products are electrophoresed on...
Violent deaths are a leading cause of mortality in the United States and research suggests American Indians and Alaska Natives (AI/AN) are disproportionately impacted compared to all other racial and ethnic groups. Little is known about the true magnitude of the serious threat of violence mortality in AI/AN communities across...