We describe a series of novel computational models, CERENKOV (Computational Elucidation of the REgulatory NonKOding Variome) and its successors CERENKOV2, CERENKOV3, and Convolutional CERENKOV3, for discriminating regulatory single nucleotide polymorphisms (rSNPs) from non-regulatory SNPs within non-coding genetic loci. The CERENKOV models are designed for recognizing rSNPs in the context of...
PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...
A Kalman filter was developed to recover information on short period variations of ocean circulation from the satellite altimeter signals for the North Pacific Ocean. The ocean circulation at each grid point is specified by three variables: sea level height h, zonal velocity u, and meridional velocity v. In our...
This study was undertaken to investigate the variation in the total sugar and acid content of the frozen Marshall strawberries grown in different areas of the Pacific Northwest. The samples used in this study were collected from different freezing plants located in the states of Oregon and Washington during the...
Shan-Mei, a Tsou aboriginal village in Taiwan, is widely known as a legend of environmental conservation, where ecotourism has been successfully combined with integrated community development. Indigenous knowledge containing "ecological wisdom" and decision-making based on consensus are perceived to be the greatest contributors to this achievement. I conducted three months...
Web-based Pesticide Screening Tool (Web-PST) is a web-based software application for evaluating the potential risk of pesticides on the surrounding environment. It uses the formulas and standards specified by the Soil/Pesticide Interaction Screening Procedure Version II (SPISP II). Web-PST closely models the stand-alone Windows application Windows Pesticide Screening Tool (WIN-PST)....
The isoprenoid pathway is one of the major biosynthetic pathways of secondary metabolites. Isoprenoids with a C₂₀ skeleton are known as diterpenoids and are derived from the common precursor, geranylgeranyl diphosphate (GGPP). This dissertation describes approaches to discover the diterpene synthase enzymes which provide the backbone structures for pleuromutilin and...
This exploratory investigation aimed to identify preservice science teachers' conceptions of the nature of science (NOS), and worldviews that represent their culturally dependent beliefs about the world, in the context of Taiwan. The interrelationships between the responses elicited from both the assessments of NOS understandings and worldviews were examined. Participants...
Polycomb Repressive Complex 2 (PRC2) is a histone methyltransferase that initiates facultative heterochromatin formation by trimethylating histone H3 on lysine 27 (H3K27me3). Mutations within its three primary subunits, KMT6, EED, and SUZ12, result in inherited diseases and sporadic cancers. In the fungus Fusarium graminearum, deletion of the gene encoding the...
PRC2 (Polycomb Repressive Complex 2) mediated gene silencing plays an important role in cell differentiation, stem cell plasticity, and tumor suppression. In fungi, it is also involved in pathogenicity. The catalytic subunit, KMT6, trimethylates lysine 27 on histone h3 (H3K27me3) at loci targeted for silencing. In animals, PRC1 and PRC2...