Article

 

Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton Public Deposited

Analytics

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/9c67wp33t

This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website:  http://www.opticsinfobase.org/ao/home.cfm. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Descriptions

Attribute NameValues
Creator
Abstract
  • We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion pumps. Using light from lasers instead of broadband sources as transmittance probe beams can be advantageous when examining optically dense, highly scattering samples such as concentrated microbial cultures. Such a laser-based approach may prove useful in shipboard studies for identifying proteorhodopsin in whole cell suspensions concentrated from seawater.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Russell A. Desiderio, Samuel R. Laney, Ricardo M. Letelier, and Stephen J. Giovannoni, "Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton," Applied Optics, 46, 7329-7336 (2007)
Journal Title
Journal Volume
  • 46
Journal Issue/Number
  • 29
Rights Statement
Funding Statement (additional comments about funding)
  • Partial support for this research was provided by T. J. Cowles.
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2012-02-18T00:34:05Z (GMT). No. of bitstreams: 1 LetelierRicardoCEOASUsingLasersProbe.pdf: 662314 bytes, checksum: 8fef8de0296c5b382f791a65a6182606 (MD5) Previous issue date: 2007-10-10
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2012-02-18T00:34:05Z No. of bitstreams: 1 LetelierRicardoCEOASUsingLasersProbe.pdf: 662314 bytes, checksum: 8fef8de0296c5b382f791a65a6182606 (MD5)

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
LetelierRicardoCEOASUsingLasersProbe.pdf 2017-07-21 Public