The Effect of Iron Limitation on the Transcriptome and Proteome of Pseudomonas fluorescens Pf-5 Public Deposited

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  • One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels.
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  • Lim CK, Hassan KA, Tetu SG, Loper JE, Paulsen IT (2012) The Effect of Iron Limitation on the Transcriptome and Proteome of Pseudomonas fluorescens Pf-5. PLoS ONE 7(6): e39139. doi:10.1371/journal.pone.0039139
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  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2012-07-20T23:52:26Z (GMT) No. of bitstreams: 3 license_rdf: 19965 bytes, checksum: 225316337756db2af069c3edfe03a49f (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) LoperJoyceBotanyPlantPathologyEffectIronLimitation.pdf: 1220033 bytes, checksum: 45084d563223a610c2bbbed0c28208f9 (MD5)
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  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2012-07-20T22:52:56Z No. of bitstreams: 3 license_rdf: 19965 bytes, checksum: 225316337756db2af069c3edfe03a49f (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) LoperJoyceBotanyPlantPathologyEffectIronLimitation.pdf: 1220033 bytes, checksum: 45084d563223a610c2bbbed0c28208f9 (MD5)

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