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Effects of megagametophyte removal on DNA yield and early seedling growth in coastal Douglas-fir

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https://ir.library.oregonstate.edu/concern/articles/wm117p48w

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  • Stratified seeds of coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. menziesii) were germinated, sown in soil, and seed coats and megagametophytes were removed at various stages of early seedling development. Yield and quality of DNA extracted from the megagametophytes were related to several morphological traits of the seedlings after 2 months of growth in a controlled environment chamber. Regression and analysis of variance demonstrated nonlinear associations between stage of megagametophyte removal and seedling size traits, DNA yield and quality, and RNA presence. Megagametophyte removal when cotyledons had extended one-quarter of their length (about 4 mm) outside the seed coat (our stage 4) resulted in sufficient DNA for construction of saturated PCR (polymerase chain reaction) based genome maps and had little effect on seedling development.
  • Keywords: DNA yield, megagametophytes, seedling size traits
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  • Krutovskii, K.V., S.S. Vollmer, F.C. Sorensen, W.T. Adams, and S.H. Strauss. 1997. Effects of megagametophyte removal on DNA yield and early seedling growth in coastal Douglas-fir. Canadian Journal of Forest Research 27(6): 964-968.
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  • 27
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  • 6
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