Graduate Thesis Or Dissertation
 

Investigating the Genetic Control of Fusarium Root Rot Resistance in Snap Beans

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/1831cp380

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  • Root rot diseases are a major constraint to bean (Phaseolus vulgaris L.) production around the world. Both snap beans and dry beans are affected. Root rot diseases can be caused by a variety of pathogens; however, Fusarium solani is a common causal agent. Fusarium root rot is a primary yield limitation of snap bean production in Oregon. Cultural control methods are ineffective and the pathogen will be present at the end of one season of production on previously clean land, indicating the need for genetic resistance. In order to address this need, a diversity panel of 148 snap bean varieties (the BeanCAP Snap Bean Diversity Panel) was evaluated for resistance to Fusarium root rot in Oregon. Morphological traits potentially involved in root rot resistance were also evaluated. Genome-wide association studies were conducted to locate SNPs associated with Fusarium root rot resistance, aboveground biomass, adventitious roots, taproot diameter, basal root diameter, deepest root angle, shallowest root angle, root angle average, root angle difference, and root angle geometric mean. Significant associations were located for all traits evaluated, including associations with root rot resistance on Pv02, Pv08, Pv09, and Pv10. Genomic estimated breeding values based on BLUPs and BLUEs were generated for each variety and trait. In order to investigate the genetic architecture of the diversity panel principal component analysis, a kinship heat map, and a neighbor-joining phylogenetic tree were generated. The first principal component axis separated varieties by center of domestication, while the second principal component axis separated European extra-fine beans from other Mesoamerican varieties. Kinship analysis demonstrated an average similarity coefficient between varieties of 0.67. The phylogenetic tree indicated two separate derivations of snap beans with extensive subsequent recombination. In this study we also converted previously published SNP markers associated with resistance to root rot diseases in snap bean into user-friendly INDEL and KASP markers to aid in the implementation of marker-assisted selection for resistance to root rot in snap bean.
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