From the Oregon Wolfe Barley to fall-sown food barley : markers, maps, marker-assisted selection and quantitative trait loci Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2z10ws434

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Understanding complex traits is a fundamental challenge in plant genetics and a prerequisite for molecular breeding. Tools for trait dissection are markers, maps, and quantitative trait locus (QTL) analysis. Marker-assisted selection (MAS) is an application that integrates these tools. In this thesis research, a new sequence-based marker was evaluated, maps were constructed and used, and QTLs were detected using two types of populations. Marker-assisted selection was used to develop a novel class of barley. Restriction-site Associated DNA (RAD), a sequence based-marker technology, allows for simultaneous high-density single nucleotide polymorphism (SNP) discovery and genotyping. We assessed the value of RAD markers for linkage map construction using the Oregon Wolfe Barley (OWB) mapping population. We compared a RAD-based map to a map generated using Illumina GoldenGate Assay (EST-based SNPs). The RAD markers generated a high quality map with complete genome coverage. We then used the RAD map to locate QTL for agronomic fitness traits. A paper describing this research was published (Chutimanitsakun et al., 2011). Marker-assisted selection was used to rapidly develop fall-sown barley germplasm for human food uses. The target traits were high grain β-glucan, vernalization sensitivity (VS) and low temperature tolerance (LTT). The target loci were WX and VRN-H2. Marker-assisted selection was effective in fixing target alleles at both loci and waxy starch led to increase in grain β-glucan. Unexpected segregation at VRN-H1 and VRN-H3, revealed by genome-wide association mapping (GW-AM), led to unanticipated phenotypic variation in VS and LTT. We found that GW-AM is an efficient and powerful method for identifying the genome coordinates of genes determining target traits. Precise information is obtained with perfect markers; additional research may be needed when multiple alleles are segregating at target loci and significant associations are with markers in linkage disequilibrium (LD) with the target loci. A paper describing this research will be submitted for publication.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2011-12-19T22:47:37Z (GMT) No. of bitstreams: 1 ChutimanitsakunYada2012.pdf: 1603401 bytes, checksum: e775a1be0ed226dc732ea38bc1f2b569 (MD5)
  • description.provenance : Submitted by Yada Chutimanitsakun (chutimay@onid.orst.edu) on 2011-12-17T01:05:52Z No. of bitstreams: 1 ChutimanitsakunYada2012.pdf: 1603401 bytes, checksum: e775a1be0ed226dc732ea38bc1f2b569 (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2011-12-19T20:53:09Z (GMT) No. of bitstreams: 1 ChutimanitsakunYada2012.pdf: 1603401 bytes, checksum: e775a1be0ed226dc732ea38bc1f2b569 (MD5)
  • description.provenance : Made available in DSpace on 2011-12-19T22:47:37Z (GMT). No. of bitstreams: 1 ChutimanitsakunYada2012.pdf: 1603401 bytes, checksum: e775a1be0ed226dc732ea38bc1f2b569 (MD5) Previous issue date: 2011-12-07

Relationships

In Administrative Set:
Last modified: 08/03/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items