Evaluation of the oscilloscope technique for detection of dormancy and survival potential of coniferous seedlings Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4m90f0023

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  • Oscilloscope display of modification of a square wave signal passed through stem tissue was evaluated as a means of detecting depth of dormancy and survival potential of coniferous seedlings. Trace form did not change during release from deep dormancy in Douglas fir. This release was judged by observing speed of flushing of lateral and terminal buds in a favorable long-day environment. In contrast, increased apical activity in the pine was signaled by trace change. In both Douglas-fir and ponderosa pine approaching spring bud-swell was well indicated by peaking of trace form. Half of the seedlings sampled from fall through spring had been subjected to moisture stress during the previous summer to hasten the onset of dormancy. Fall trace forms of the stressed pine indicated this change. Moisture stress did not change dormancy patterns in the fir. Date of cambial growth cessation in the fir and pine was determined using Wolter's camb.ial scarring technique. Diameter growth ceased during the period in which trace form changed greatly. Cambial growth cessation has been linked to cold acclimation; this and other evidence suggest that the oscilloscope technique could be used to detect frost hardiness. The second part of this research was a direct test of the ability of the oscilloscope technique to detect survival potential of Douglas-fir seedlings lifted on six dates from October 11 through March 24, and planted after zero, four, or eight weeks of cold storage. On a site with moderate moisture stress, trace form at time of lifting indicated potential survival of stored Douglas-fir seedlings. Survival of mid-October and late March lifted seedlings was low. These seedlings had peaked traces when lifted. Seedlings from the middle lifting dates had nearly square traces; survival of these seedlings was excellent. Growth performance of November lifted seedlings with square traces was poor, however. On a more severe site, these seedlings may not have survived. A square trace form at time of lifting is not, therefore, necessarily a guarantee of survival. Correlation of trace form after storage with survival and growth parameters was poor. Trace character is apparently not capable of detecting vigor per se but only seasonally related physiological factors.
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