Graduate Thesis Or Dissertation
 

Optimizing virus detection in potato breeding populations

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5425kc97n

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  • Virus contamination in a potato breeding program can seriously disrupt the variety development process. Experiments were conducted from 1989 through 1993 to: evaluate the extent to which Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY) can invade a breeding program, determine viral spread within hills and within individual tubers, and compare viral detection methods. Enzyme-linked immunosorbent assay (ELISA) of tuber sap for PLRV was most accurate when samples were taken from stem end eyes. Virus titer levels decreased from stem to bud ends. False negative tests were sometimes frequent with tuber testing, but false positives were rare. ELISA of grow-out plants was more accurate than tuber sap ELISA for detecting PLRV. Testing accuracy was further improved by growing plants from tuber bud end eyes because of improved emergence and survival. Visual inspection of grow-out plants was less effective than either foliar or tuber ELISA for determining the presence of PLRV. PLRV free tubers were detected within infected hills. However, no disease free eyes were found in infected tubers. PLRV titer levels decreased with storage duration, but detectability of infected tubers was unaffected. Tuber testing (sap ELISA) for PVY was most accurate using eyes from tuber bud ends. PVY titer levels varied within tubers. Tuber testing for PVY generated many erroneous results, both positive and negative. As with PLRV, foliar ELISA of grow-out plants was the preferred method for detecting PVY in tubers. Visual inspection of daughter plants for PVY symptoms was often ineffective. PVY-free tubers were found in infected hills, and unlike PLRV, healthy tissues were sometimes isolated from infected tubers. Both the titer level and detectability of PVY in tubers declined with time in storage. Studies of casual handling of greenhouse grow-out plants showed PVY movement between adjacent plants. However, spread was caused by foliar contact between adjacent plants and not by handling. PVY spread was not visually evident, but was detectable with ELISA.
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