Graduate Thesis Or Dissertation
 

Studies on the effect of moderate temperature on Vibrio marinus

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  • Washed cells of Vibrio marinus MP-1 grown at 15 C (organism's optimum growth temperature) were employed in this study. These cells were exposed to heat at and above their maximal growth temperature (20 C) for one hour and tested manometrically at 15 C for their ability to take up oxygen. Endogenous activity, as evidenced by oxygen uptake, decreased with increased temperatures of exposure and was destroyed at 30 C. The same pattern of oxygen uptake was noted when glucose was employed as the substrate. Oxygen uptake decreased with increasing exposure periods at temperatures above the maximum growth temperature. Endogenous oxygen uptake was negligible after exposure at 29.2 C for 50 minutes. The supernatants, which resulted from suspensions of cells exposed to 15 to 35 C, contained 260-280 mμ absorbing material. This increased with increased temperature as well as increased time of exposure. The most radical changes occurred above 28 C. Supernatants were further analyzed for Kjeldahl nitrogen, orcinol reacting material and diphenylamine reacting material. The latter are indicative of ribonucleic and deoxyribonucleic acid respectively. The data indicates that moderate temperatures from 20 to 30 C are sufficient to inactivate the metabolic systems involved in oxygen uptake both endogenously and in the presence of glucose. Also, cellular composition and permeability are affected, as evidenced by the leakage of 260-280 mμ material.
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