Graduate Thesis Or Dissertation
 

Quantification of fructose equivalents in straw extracts

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/hm50tt966

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  • Cereal and commercial grass-seed straws are abundant plant biomass materials that are currently being considered for application in industrial processes aimed at the production of biofuels, biomaterials, and biochemicals. The carbohydrate fraction of these straws is of particular interest in this context due to its chemical functionality and relative ease of fermentation. Thus there is a global effort to develop economical methods through which the carbohydrate fractions of straws may be obtained in a readily utilizable form. The research presented in this thesis is designed to contribute to that effort by determining appropriate conditions for the analysis of the fructose-equivalent content of water extracts of straw. The basis of the method is to hydrolyze the parent fructose-containing poly/oligosaccharides and then quantitatively measure the resulting fructose. The focus of the study is the hydrolysis, which is to be done using conditions similar to those used for the analysis of the other neutral sugars in biomass materials. Experiments were done using model fructans and water extracts from a representative wheat straw and Tall Fescue straw. The results demonstrate that the standard conditions used for the analysis of raw biomass (minimum acid concentrations for hydrolysis being 4% H₂SO₄) are too severe for the quantification of fructans; under these conditions greater then 50% of the fructose is degraded. It is shown that fructans are effectively hydrolyzed at acid concentrations as low as 0.1%. Application of the method demonstrates that fructose makes up a significant percentage of the total neutral sugars extracted with hot water from either of these straws.
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