Graduate Thesis Or Dissertation
 

Laboratory investigations of the natural fluorescence of marine phytoplankton

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  • The influence of nitrate availability and irradiance on phytoplankton natural fluorescence was investigated in laboratory cultures of the marine diatom Thalassiosira weissflogii (Bacillariophyceae). Two cultures of phytoplankton, differing only in the nitrate-limited growth rate, were compared to learn how increases in irradiance influences natural fluorescence. Instrumentation was developed for these experiments to maintain and manipulate physical and chemical conditions in the culture vessel while unobtrusively and continuously monitoring the natural fluorescence response. Variable fluorescence in each culture was simultaneously measured to provide a concurrent data set of photosynthetic physiology. Analysis of results from these experiments suggest that gross features in the relationship between fluorescence and irradiance contain information about the way in which phytoplankton perceive the relative abundance of environmental nitrate. This relationship becomes less informative at higher irradiance levels due to the increasing influence of photoinhibition and photoprotection.
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file details LaneySamuelR2001.pdf 2017-08-19 公开 下载