Graduate Thesis Or Dissertation
 

Zinc-65 uptake by a bacterium isolated from Alder Slough, Columbia River Estuary

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  • Bacteria were isolated from water at Alder Slough, Oregon. Of 15 isolates grown successfully in the medium employed, 100% showed measurable uptake of Zn-65. A growth curve was established for one isolate, a gram-negative rod designated AS-1. Increasing Zn-65 uptake was found generally to correspond with increasing growth of AS-1. The optimum growth temperature for this isolate was 31°C, with very little growth at 37.5°C and 6.7°C. Growth and Zn-65 uptake occurred in a 35 ppt medium from a temperature of 31.0°C to 13.9°C but little growth and no Zn-65 uptake was observed at 8.6°C and 4.4°C. Growth and Zn-65 uptake in a 0 ppt medium occurred from 31.0°C to 5.0°C, with little growth but measurable Zn-65 uptake at 8.9°C and 5.0°C. It was found that 200 ppm Mg added to a culture which had already taken up Zn-65 failed to displace the zinc from the cells. A interaction of the energy source, Casamino Acids, with the Zn-65 spike influenced the results. When cells were lysed, spiked with Zn-65, and the debris removed by centrifugation, 84.8% of the Zn-65 remained in the supernatant. But when cells grown first in Zn-65 spiked medium were lysed and centrifuged, 74.4% of the Zn-65 was found in the debris. It was concluded that bacterial uptake of Zn-65 and other metal cations must be considered in determining the fates of these materials released into the environment. Chemical adsorption phenomena were determined to be a major factor controlling this uptake, with other chemical and biological factors, such as competitive binding by the medium and active uptake or exclusion by the cells, exerting a significant influence that requires further investigation to characterize.
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