A study of the fatty acids and lipids of Clostridium botulinum type B Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/w95054053

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  • The difficulties encountered in working with micro-quantities of lipids have been overcome by the advent of new and improved procedures in chromatography which have made possible better fractionation and identification. Bacterial lipids because of their different lipid pattern from other life forms have stimulated much interest. Studies have been made on the nature of the various lipid complexes, its synthesis and degradation, its distribution intra-cellularly, its immunological properties and functions. The purpose of this investigation was to determine the lipid and fatty acid composition of the vegetative cells of Clostridium botulinum 115B as an initial step to the understanding of its high resistance to radiation as well as heat and to its toxigenicity. Lyophilized cells were used for extraction. Free fatty acids were separated from total fatty acid by column chromatography and analyzed for their fatty acid compositions by gas-liquid chromatography after conversion into methyl esters. Volatile fatty acids were analyzed using GLC, as free acids. The total lipid was resolved into its component lipid classes by thin-layer chromatography. Analysis showed that hydrocarbons, di- and tri-glycerides, free fatty acids, phospholipids and glycolipids make up the lipid composition. The overall fatty acid pattern included straight chain saturated, unsaturated and cyclopropane acids with a complete spectra from C₁ - C₂₁. Normal saturates make up the major portion of the fatty acids with palmitic acid being the most predominant. Normal unsaturated acids comprised the second major group which consisted mostly of monoenoic acids, the most abundant of which was an octadecenoic acid.
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