Honors College Thesis
 

Thermodynamic Design Principles for Double-Strand Displacement microRNA Biosensors

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  • In recent years, microRNA’s (miRNA’s) have emerged as important in regulating gene expression. Fluctuations in their levels are associated with the up or down-regulation of tumor-suppressor genes and oncogenes. Thus, the ability to visualize changes in miRNA is an important step in understanding cancer emergence and development. The Burrows group has developed a novel double-strand displacement biosensor with high sensitivity that mitigates false signals caused by endonuclease degradation. The selectivity of the biosensor for a particular miRNA is determined by the nucleic acid sequence of the reporter. This work is focused on the development of a method to select a reporter that will yield the best selectivity. In particular, I’ve focused on selecting a reporter for miR-29b-1-5p. Variations in the expression levels of this miRNA are associated with lung cancer, cervical cancer, and lymphocytic leukemia. The principles outlined in this work can be extended to select a reporter for any miRNA utilizing the double-strand displacement biosensor design. Key Words: Biosensor, double-strand displacement, reporter-probe complex, probe-analyte complex, hairpin conformation, quenching, micro-ribonucleic acid (miRNA), reporter selection, miR29b-1-5p
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