Honors College Thesis
 

Measuring Anti-Müllerian Hormone Concentrations to Determine Reproductive Status in Female Rabbits

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  • There are over two million pet rabbits in the United States. Many rabbits are obtained as adults from pet stores and humane societies. Pet rabbit owners may elect to perform ovariohysterectomy (spay) surgeries on female rabbits to prevent unwanted pregnancies as well as to eliminate the possibility of reproductive disorders (e.g., uterine cancer). Unfortunately, there is no reliable method currently available for determining if a female rabbit has already been spayed. Anti-Müllerian hormone (AMH) is produced by granulosa cells within ovarian follicles. In cats, the presence AMH can be used to differentiate between intact and spayed females. The purpose of this thesis research was to determine if AMH is reliable in determining reproductive status in rabbits. Blood samples were collected from adult female rabbits (10 intact; 7 spayed) during routine laboratory testing and remaining sera was donated for use in this research. Anti-Müllerian hormone concentrations were measured by a commercially available enzyme-linked immunosorbent assay (ELISA) designed for rabbits, and results were quantified on a plate reader. In addition, each well was examined visually in comparison to known standards to determine reproductive status. From the visual assessment, sensitivity and specificity were calculated. The optical densities of all but one sample were above the upper limit of this ELISA for estimating AMH concentrations. Although the specificity was moderately high (80%), the sensitivity was low (11%), indicating that this assay would not be suitable for determining reproductive status in female rabbits.
  • Key Words: anti-Müllerian hormone, enzyme-linked immunosorbent assay, Müllerian-inhibiting hormone, ovariohysterectomy, rabbit, spay
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  • This thesis research was funded by URSA Engage.
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