Larkin_seminar presentation-1.pdf

Undergraduate Thesis Or Project

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/s7526f26x

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Attribute Name	Values
Creator	Larkin, Andrew Siddens, Lisbeth Krueger, Sharon
Abstract	Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin to limit degradation. Potential sources of RNA degrdation include RNase activity, both endogenous and exogenous, thermal degradation during heatinduced epidermal-dermal seperation, and contamination of stock solutions. Testing of the modified method produced dermal RNA samples with acceptable RIN integrity levels (RIN mean 7.6). Variations of the modified method were performed and compared to one another for method optimization. Keywords: dermal RNA, environmental PAH mixtures, FVB/N mice, epidermal RINs Keywords: dermal RNA, environmental PAH mixtures, FVB/N mice, epidermal RINs
Contributor	Krueger, Sharon Williams, David E. Baird, William M. Siddens, Lisbeth K.
Rights Statement	Copyright Not Evaluated

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