Index Catalog // ScholarsArchive@OSU

Synthesis and function of bioactive, block copolymer surfactant constructs as relevant to the preparation of anticoagulant and antibacterial medical implant surfaces.

Creator:: Joshi, Pranav R.
Abstract:: Nisin, an antibacterial peptide proven to be an effective inhibitor of Gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. Covalent coupling was achieved by chemical modification of the N-terminal isoleucine to introduce a thiol group. Thiolated nisin derivatives were then linked to poly[ethylene...
Resource Type:: Dissertation
Full Text:: depolymerized by chemical degradation using nitrous acid, or enzymes from Flavobacter to yield fragments of

Nisin adsorption and function at hydrophobic surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock surfactant Pluronic® F108

Creator:: Tai, Yuan-Ching
Abstract:: The adsorption and elution of the antimicrobial peptide nisin at hydrophobic, silanized silica surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]- poly[ethylene oxide] surfactant Pluronic® F108 was measured in situ, with ellipsometry. While such layers are known to inhibit protein adsorption, nisin was observed to adsorb in multilayer quantities, to an...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE DISSERTATION OF Yuan-Ching Tai for the degree

Functionalization of Surfaces with Nisin in a Polyethylene Oxide Brush Layer

Creator:: Ryder, Matthew P.
Abstract:: Infections in hospitals account for over 100,000 deaths per year. These infections occur at the hospital from complications following bacterial adhesion to intravenous catheters, coronary stents and other implanted devices. Another common problem is protein adsorption to the surface of the device and subsequent blood clotting. Methods for combating these...
Resource Type:: Honors College Thesis
Full Text:: physiological conditions. Tai’s work investigated the brush layer in a solution of low ionic strength (10 mM

The adsorption of human recombinant factor VIII in the presence of the nonionic triblock surfactant Pluronic® F-68 at the air-water interface

Creator:: Alkhatib, Aveen K.
Abstract:: The adsorption behavior of a human recombinant Factor VIII (rFVIII) in the presence of the nonionic, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock surfactant Pluronic® F-68 was evaluated by dynamic air-water tensiometry. Surface tension kinetics were recorded for F-68 in the absence of protein at concentrations ranging from 0 to 10,000...
Resource Type:: Masters Thesis
Full Text:: rapid and will cause degradation of the protein leading to a loss of activity. rFVIII activity loss

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

Creator:: Lee, Hyo Jin
Abstract:: Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and...
Resource Type:: Masters Thesis
Full Text:: different properties. On hydrophobic surfaces, the binding strength depended on the structural rigidity of

Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects

Creator:: Joshi, Omkar
Abstract:: Adipic dihydrazide-modified heparin was prepared and subsequently immobilized on surface-activated silica wafers. X-ray photoelectron spectroscopy (XPS) was used to quantify the elemental composition of the silica surface at each stage of activation and results showed that successful immobilization had taken place. Contact angle measurements were made to verify the high...
Resource Type:: Masters Thesis
Full Text:: , such as the rate of adsorption, strength of binding and conformational state of adsorbed proteins

Surfactant interactions with recombinant factor VIII at the air/water interface

Creator:: Chu, Liping
Abstract:: The surface tension kin'ti4tnd ste'dy state behavior exhibited by mixtures of selected surfactants with a recombinant Factor VIII (rFVIII) were evaluated with automated interfacial tensiometry. In particular, fresh solutions of rFVIII with different concentrations of Tween 80, sodium dodecylsulfate (SDS), or dodecyltrimethylammonium bromide (DTAB) were prepared and their surface tension...
Resource Type:: Masters Thesis
Full Text:: phenomenon called surface denaturation. Green, Hopkinson and Jones (1998) studied the process of thermal

Molecular properties influencing the surface activity of α-lactalbumin, β-lactoglobulin and bovine serum albumin

Creator:: Suttiprasit, Prasert
Resource Type:: Dissertation
Full Text:: Albumin Abstract approved: 7 /I#r. Joseph McGuire The thermal stability of a-lactalbumin

Elution of adsorbed proteins at hydrophobic and hydrophilic surfaces by dodecyltrimethylammonium bromide and sodium dodecyls sulfate

Creator:: Vinaraphong, Pravina
Abstract:: Adsorption kinetic data recorded for α-lactalbumin, β-casein, β-lactoglobulin, bovine serum albumin and lysozyme at silianized silica surfaces of low and high hydrophobicity, along with a simple model for adsorption and surfactant-mediated elution of protein, were used to analyze the removal of each protein by sodium dodecylsulfate (SDS) and dodecyltrimethylammonium bromide...
Resource Type:: Masters Thesis
Full Text:: surfaces. They found that the strength of protein- surface binding increased slowly over a period of days

Adsorption of selected charge mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Creator:: Podhipleux, Nilobon
Abstract:: The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution resulted in a decrease in the net charge of the protein by 2 units....
Resource Type:: Masters Thesis
Full Text:: Adsorptions 3 2.2 Effects of pH, Ionic Strength, and Charge 6 3. THEORY 12 3.1 Basic Features of

The influence of surface functional groups on β-lactoglobulin adsorption equilibrium

Creator:: Al-Makhlafi, Hamood K.
Abstract:: Interactions between proteins and contact surfaces can have important implications in the food industry. Such interactions contribute to the course of fouling of membrane surfaces and they appear to mediate bacterial and spore adhesion to some degree as well. In addition to protein and solution properties, interfacial behavior is strongly...
Resource Type:: Masters Thesis
Full Text:: quantitative indication of the strength of interaction between a protein molecule and a solid surface

Use of contact angle analysis for the measurement of the relative hydrophilicity of food contact surfaces

Creator:: Kirtley, Sidney A.
Resource Type:: Masters Thesis
Full Text:: research is needed to determine the effects of hydrophobic interactions on the strength of adhesion

The influence of preadsorbed milk proteins on adhesion of Listeria monocytogenes to silica surfaces

Creator:: Al-Makhlafi, Hamood K.
Abstract:: β-lactoglobulin (β-Lg), bovine serum albumin (BSA), α-lactalbumin (α-Lac), and β-casein were adsorbed onto silanized silica surfaces of low and high hydrophobicity for 8 h, and β-Lg and BSA for 1 h. The surfaces were incubated in buffer for 0, 5, 10, or 15 h and then contacted with Listeria monocytogenes...
Resource Type:: Dissertation
Full Text:: proportional to the square root of the ionic strength (59). Hence at high electrolyte concentration, or in

Interaction of recombinant factor VIII and the nonionic surfactant Tween 80 at interfaces

Creator:: Joshi, Omkar
Abstract:: The role of the nonionic surfactant Tween 80 on the behavior of the therapeutic recombinant protein Factor VIII (rFVIII) was investigated at solid/liquid and air/water interfaces. In order to provide a model system to compare results obtained for the complicated rFVIII-Tween system, a well-characterized globular protein lysozyme was used. The...
Resource Type:: Dissertation
Full Text:: ....................... 59 3.8 Effect of ionic strength on the adsorption of rFVIII 8 ppm Tween on a hydrophilic surface

Assaying the activities of Thermomonospora fusca E₅ and Trichoderma reesei CBHI cellulases bound to polystyrene

Creator:: Kongruang, Sasithorn
Abstract:: In this study the enzymatic activity of adsorbed Thermomonospora fusca E₅ and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential...
Resource Type:: Masters Thesis
Full Text:: degradation of cellulose chains into glucose residues by a system of cellulases and 13-glucosidase. Enzymatic

Kinetic modeling of the adsorption of structural stability mutants of bacteriophage T4 lysozyme at solid-water interfaces

Creator:: Lee, Woo-Kul
Resource Type:: Dissertation
Full Text:: rate and extent of secondary structure loss were related to protein thermal stability, with greater

Molecular origins of surfactant-mediated stabilization of protein

Creator:: Lee, Hyo Jin
Abstract:: Nonionic surfactants are commonly used to stabilize proteins during upstream and downstream processing and drug formulation. Surfactants stabilize the proteins through two major mechanisms: (i) their preferential location at nearby interfaces, in this way precluding protein adsorption; and/or (ii) their association with protein into "complexes" that prevent proteins from interacting...
Resource Type:: Dissertation
Full Text:: (potentially irreversible)3 10 affinity was dependent on the buffer ionic strength and the type of anion

Adsorption of the wild type and a synthetic structural stability variant of bacteriophage T4 lysozyme

Creator:: Podhipleux, Nilobon
Resource Type:: Dissertation
Full Text:: properties of the adsorbing protein, than by the conformational state and binding strength of the already

Structural stability effects on adsorption of bacteriophage T4 lysozyme to colloidal silica

Creator:: Tian, Minghua
Abstract:: Circular dichroism (CD) spectra were obtained for bacteriophage T4 lysozyme and three of its mutants in the presence and absence of colloidal silica nanoparticles. Mutant lysozymes were produced by substitution of the isoleucine at position 3 with tryptophan, cysteine and leucine. Each substitution resulted in an altered structural stability, quantified...
Resource Type:: Masters Thesis
Full Text:: that proteins of lower thermal stability have a greater tendency to adopt state 2 on silica

Structural effects on enzymatic activity of bacteriophage T4 lysozyme upon adsorption to colloidal silica

Creator:: Xu, Qiurong, 1964-
Resource Type:: Masters Thesis
Full Text:: strength of solution, solute type and concentrations along with the motion of fluid also play an important

Sequential and competitive adsorption of BSA and β-lactoglobulin, and their resistance to exchange with [sigma]-lactalbumin and β-casein

Creator:: Nasir, Adil
Resource Type:: Masters Thesis
Full Text:: ). Calcium binding, which may stabilize thermal denaturation, is another characteristic of a-lactalbumin

Resistance of adsorbed nisin to exchange with bovine serum albumin, α-lactalbumin, β-lactoglobulin, and β-casein at silanized silica surfaces

Creator:: Muralidhara, Lakamraju
Abstract:: Nisin is an antibacterial peptide, which when adsorbed on a surface can inhibit bacterial adhesion and viability. The ability of noncovalently immobilized nisin to withstand exchange by the milk proteins bovine serum albumin, β-lactoglobulin, α-lactalbumin, and β-casein on surfaces that had been silanized with dichlorodiethylsilane to exhibit high and low...
Resource Type:: Masters Thesis
Full Text:: constant, the data suggested that the binding strength of the adsorbed protein had changed over time

Macroscopic model for apparent protein adsorption equillibrium at hydrophobic solid-water interfaces

Creator:: Al-Malah, Kamal Issa Masoud
Resource Type:: Dissertation
Full Text:: . 30) 48 4.2 Thermal properties of proteins used in evaluating AGmfdd (equation 13), with

Adsorption of synthetic stability mutants of bacteriophage T4 lysozyme at silanized silica surfaces

Creator:: Singla, Brijesh
Resource Type:: Masters Thesis
Full Text:: lysozyme contains one less disulfide bond, and is of lower thermal stability than hen lysozyme, and by

β-Lactoglobulin adsorption equilibrium at low- and high-energy surfaces

Creator:: Al-Malah, Kamal Issa Masoud
Abstract:: Ellipsometry was used to study the effects of surface energetics and temperature on the equilibrium adsorptive behavior exhibited by β-lactoglobulin. β-Lactoglobulin isotherms at 25, 37, and 55°C were constructed for this purpose. The surfaces of acrylic, polycarbonate, polyester, glass, and #304 stainless steel were contacted with protein solutions of varying...
Resource Type:: Masters Thesis
Full Text:: of ionic strength in sodium phosphate buffer. Removal of tripalmitin from a PVC surface by means

Molecular origins of peptide entrapment in polyethylene oxide layers

Creator:: Wu, Xiangming
Abstract:: A more quantitative understanding of peptide entrapment and elution from otherwise protein-repellent polyethylene oxide (PEO) brush layers will provide direction for development of new strategies for drug storage and delivery. Here we describe criteria for peptide integration and structural change within the PEO brush, and discuss the reversibility of peptide...
Resource Type:: Dissertation
Full Text:: Molecular Origins of Peptide Entrapment in Polyethylene

Binding of bacterial lipopolysaccharide by the cationic amphiphilic peptide WLBU2 at interfaces

Creator:: Ryder, Matthew P.
Abstract:: Passage of blood through a sorbent device for removal of bacteria and endotoxin by specific binding with immobilized, membrane-active, bactericidal peptides holds promise for treating severe blood infections. Peptide insertion in the target membrane and stable binding is desirable, while membrane disruption and release of degradation products to the circulating...
Resource Type:: Dissertation
Full Text:: , while membrane disruption and release of degradation products to the circulating blood is not

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