Graduate Thesis Or Dissertation
 

Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2j62s713j

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  • Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and showed that the heparinization was successful. Adsorption time effects on human plasma fibrinogen adsorption at each type of surface were studied by in situ ellipsometry. Less fibrinogen adsorption and a slower adsorption rate were observed for heparinized silica relative to unheparinized silica in all cases. The history dependence of adsorption was examined by recording the sequential adsorption of fibrinogen. Differences between the rates of the first and the second adsorption step recorded at identical initial values of adsorbed mass indicated structural rearrangement of the molecules on the surface. Increased adsorption time in the first adsorption step led to more structural rearrangements, with the effect of history dependence observed to be more pronounced on the heparinized silica. It is believed that these rearrangements involve fibrinogen aggregate formation at the interface, presumably facilitated on heparinized silica by enhanced molecular mobility.
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