Index Catalog // ScholarsArchive@OSU

Enhanced Protein Homeostasis Mechanisms in Naked Mole Rats Cells

Creator:: Pride, Harrison, Coles, Alex, Mochnick, Jillian, Zhang, Yiqiang, Buffenstein, Rochelle, Hornsby, Peter J., Austad, Steve, Richardson, Arlan, and Perez, Viviana I.
Abstract:: Phylogenic studies suggest that cells from long-lived species are more resistant to a variety of stressors than short-lived species. However, there is little information on the cellular mechanisms that give rise to increased resistance to stress. Our previous studies have shown that liver proteins from a long-lived species have lower...
Resource Type:: Poster

Kinetic studies of the oxygen requirement of some amine oxidases

Creator:: Swindell, Robert Malcolm
Abstract:: Some kinetics of the oxygen requirement of amine oxidases have been investigated using bovine plasma monoamine oxidase (PAO) and pea seedling diamine oxidase (DAO). A system is described for studying enzyme reactions under controlled oxygen tension and in an O₂-free N₂ atmosphere. O₂ Km values were determined at two different...
Resource Type:: Masters Thesis
Full Text:: -4 to 10 -6 M depending on the enzyme and amine present in the reaction mixture. Ratios of moles

The purification and characterization of the fructose-1, 6-diphosphate aldolase from Bacillus stearothermophilus (NCA 2184)

Creator:: Quinn, Richard Paul
Abstract:: The thermophilic aldolase from Bacillus stearothermophilus (NCA 2184) has been purified to a specific activity of 45.5 μ-moles of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme, representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation and by a specific...
Resource Type:: Dissertation
Full Text:: in the presence of sub- strate.strate. The K (1.1 x 10 M) and Vmax (4650 moles of substratem

A double-stranded secondary structure for hyaluronic acid in aqueous-organic solvent as revealed by vacuum ultraviolet circular dichroism

Creator:: Staskus, Paul
Abstract:: The association of hyaluronic acid (HA) into a double stranded structure has been investigated by circular dichroism (CD) spectroscopy into the vacuum ultraviolet region. The CD of HA changes dramatically, monitoring a cooperative transition as the dielectric constant of an aqueous solution is reduced by adding organic solvents. This structural...
Resource Type:: Dissertation
Full Text:: THE THESIS OF Paul Staskus for the degree of Doctor of Philosophy in Biochemistry and Biophysics

The conversion of some monoalkylhydrazines to their corresponding hydrocarbons by a hepatic microsomal enzyme system in rat

Creator:: Prough, Russell A.
Abstract:: An aspect of the metabolism of monoalkylhydrazines has been investigated using both intact rats and rat liver microsomal enzyme preparations. Intact rats respired propane and n-butane on administration of 0.3-0.5 mmoles of isopropyl- and n-butylhydrazine, respectively. Based upon dose administered, a five to ten percent yield of hydrocarbon was obtained....
Resource Type:: Dissertation
Full Text:: aspect of the metabolism of monoalkylhydrazines has been investigated using both intact rats and rat

Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

Creator:: Malencik, Dean Anthony
Abstract:: Fluorescence polarization measurements on beef liver glutamate dehydrogenase conjugated with pyrene butyric acid demonstrate that the association of the enzyme is adequately described by a reversible indefinite association with a single equilibrium constant. The data obtained with protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7....
Resource Type:: Dissertation
Full Text:: dilution. The labeling varied between 0.15 and 0.4 moles PBA/mole protein. The range in points encompasses

The effect of hexachlorophene and related compounds on torula yeast glucose-6-phosphate dehydrogenase and some other dehydrogenase enzymes

Creator:: Wang, Jun-Lan
Abstract:: Low concentrations of hexachlorophene (HCP) inhibit a number of pyridine nucleotide-linked dehydrogenase enzymes. The I₅₀ HCP concentrations were 105 μM for pig heart isocitrate dehydrogenase (ICD), 65 μM for horse liver alcohol dehydrogenase, 39 μM for torula yeast glucose-6-phosphate dehydrogenase (G6PD), 6.0 μM for beef heart malate dehydrogenase, and 1.6...
Resource Type:: Dissertation
Full Text:: gradient ultracentrifugation and electrophoresis. Maximum binding of 52 moles of HCP to each mole of G6PD

Biochemical, molecular and physiological characterization of 1-butanol dehydrogenases of Pseudomonas butanovora in butane and 1-butanol metabolism

Creator:: Vangnai, Alisa S.
Abstract:: Butane-grown Pseudomonas butanovora oxidized butane by a soluble butane monooxygenase through the terminal pathway yielding 1 -butanol as the predominant product. Alcohol dehydrogenases (ADHs) involved in butane oxidation in P. butanovora were purified and characterized at the biochemical, genetic and physiological levels. Butane-grown P. butanovora expressed a type I soluble...
Resource Type:: Dissertation
Full Text:: purified to near homogeneity and characterized. BDH is a monomer of 66 kDa consisting of one mole of

The β-glucosidase system of the thermophilic fungus Chaetomium thermophile

Creator:: Lusis, Aldons Jekabs
Abstract:: The thermophilic fungus Chaetomium thermophile was shown to contain three major β-glucosidases, a cell-bound cellobiase, a cellbound aryl-β-glucosidase and an extracellular aryl-β-glucosidase. The enzymes were partially purified and studied with respect to thermal stability and certain kinetic parameters. Two of the β-glucosidases are fairly stable at the maximum growth temperature...
Resource Type:: Dissertation
Full Text:: large tyrosine and tryptophan content and an energy activationof 9. 4 Kcal per. mole. The p

Specific enzymic interactions relating to the mechanism of action on certain 2-chloroethyl nitrosoureas

Creator:: Babson, John Robert
Abstract:: Certain 2- chioroethyl nitrosoureas have been demonstrated to be highly specific inactivators of chymotrypsin in vitro and glutathione reductase, both in vivo and in isolated rat hepatocytes. The inactivation of glutathione reductase in isolated hepatocytes resulted in an increased sensitivity toward an adriamycin induced oxidative challenge. Prolonged incubation of 1-(2-chloroethyl)-3-([1-¹⁴...
Resource Type:: Dissertation
Full Text:: complementarity between the inhibitor mole- cule and the binding site at or near the catalytic site of the

Kinetics of synthesis and degradation of chicken liver xanthine dehydrogenase

Creator:: Epstein, Arnold
Abstract:: The role of synthesis and degradation in controlling enzyme concentrations was studied. Concentrations of chloride and arginine were determined for blood and liver at various stages of development from the embryo to the 8 day old chicken. The chloride concentrations were used to obtain the volume of extracellular blood in...
Resource Type:: Dissertation
Full Text:: x 10 12 and. 0.8x 10-12 moles XDH per minute per gram of liver for the 8 day chick, 1 day chick and

Biochemistry Free and Easy

Creator:: Ahern, Kevin and Rajagopal, Indira
Resource Type:: Book
Full Text:: + and 0.1 moles of Cl-. There will be no remaining HCl when this happens. A strong base like NaOH

Studies on the inhibition of bovine plasma amine oxidase by hydrazines

Creator:: Hucko-Haas, Jacqueline Elizabeth
Abstract:: Several aspects of the potent inhibition of bovine plasma amine oxidase (PAO) by hydrazines were investigated by kinetic and preparative means. The inhibition was classified as pseudo-irreversible by the method of Ackerman and Potter (Proc. Soc. Exptl. Biol. Med. 72, 1 (1949), and was found to exhibit Zone B kinetic...
Resource Type:: Dissertation
Full Text:: mole ratio, UDMH/E, was 9. 66 in both experiments and substrate concentration was 3. 33 mM. In

Hydrogen-stimulated CO₂ fixation and coordinate derepression of hydrogenase and ribulosebisphosphate carboxylase in a H₂-uptake positive strain of Rhizobium japonicum

Creator:: Simpson, Frank Berry
Abstract:: H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
Resource Type:: Masters Thesis
Full Text:: ribulose 1,5-bisphosphate to form two mole- cules of 3-phosphoglycerate and the oxygenation of RuBP to

Selenomethionine and methionine in in vitro rabbit reticulocyte hemoglobin synthesis and in Escherichia coli tRNA aminoacylation

Creator:: McCoy, Kim Edward Monroe
Abstract:: Selenomethionine and methionine have been postulated to be similarly involved in protein synthesis. This thesis has evaluated this hypothesis from a molecular and genetic approach. Selenomethionine and methionine were incorporated into rabbit reticulocyte hemoglobin at the same rates and in equal quantities. Each methionine demonstrated competitive inhibition for its analog...
Resource Type:: Dissertation
Full Text:: Selenomethionine and methionine in in vitro rabbit

Reactions of protein with phenols and quinones evaluation of amino acid modification and protein digestibility

Creator:: Wehr, Herbert Michael
Abstract:: Plant tissues contain a wide variety of phenolic compounds, frequently in high concentration. Both non-covalent association of intact and oxidized phenols and covalent linkage of oxidized phenols to protein occur. Such interactions can have important biochemical and nutritional significance. A model system approach was used to identify and quantitate amino...
Resource Type:: Dissertation
Full Text:: Reactions of protein with phenols and quinones evaluation

In vivo phosphorylation of phosphofructokinase at a novel site

Creator:: Harrahy, John J.
Abstract:: This thesis examines the interconnection between the in vitro and in vivo phosphorylation of rabbit muscle phosphofructokinase. The first goal of the project was to show whether a novel site of rabbit muscle phosphofructokinase that is subject to in vitro phosphorylation, serine 376, may also become phosphorylated in vivo. Evidence...
Resource Type:: Dissertation
Full Text:: to 0.7 moles phosphate per mole of enzyme (Luther and Lee, 1986). The effects of F-actin on the

The effect of coenzyme on the bis(1-anilino-8-naphthalenesulfonate) induced association of lactic dehydrogenase

Creator:: Lu, Fung-jou
Resource Type:: Masters Thesis
Full Text:: ir (moles of dye bound per mole of protein) to decrease froth 10.7 to 7. The concurrent release of

The interaction of polylysine with DNA as studied by polarization of flurescence

Creator:: Evett, Jay Fredrick
Abstract:: A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
Resource Type:: Dissertation
Full Text:: can be in just one of two states. In one state the labeled mole- cule is free in solution and in the

Protein methylation at sites of blood vessel injury

Creator:: Weber, Darin J.
Abstract:: Blood vessel injury was found to release intracellular pools of protein D-aspartyl/L-isoaspartyl carboxyl methyltransferase (PIMT) into the extracellular milieu, where it became trapped. Trapped PIMT was able to utilize radiolabeled S-adenosyl-L-methionine (AdoMet) introduced into the circulation to methylate blood vessel proteins containing altered aspartyl residues specifically at the site of...
Resource Type:: Dissertation
Full Text:: from Blood Vessels 67 4.1 Mole % of Selected Amino Acids in CNBr-Soluble and Insoluble Fractions 97

Binding studies of rabbit muscle phosphofructokinase : I. Binding of ATP and of 1,N⁶-ethenoadenosine-triphosphate to phosphofructokinase. II. Binding and activation of phosphofructokinase by F-actin

Creator:: Liou, Ruey Shyan
Resource Type:: Dissertation
Full Text:: the catalytic site, and the "loose" one, regulatory site. For heart phosphofructokinase, 3.6 moles

Isoprenoids of bovine aorta : isolation, purification, and biosynthesis

Creator:: Adams, Gerald Springer
Resource Type:: Dissertation
Full Text:: cholesterol, yield- ing two moles of CO2. Gautschi and Bloch later (53) definitely identi- fied this

The binding of ethidium bromide to chromatin : model for carcinogen interactions

Creator:: McMurray, Cynthia T.
Abstract:: Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
Resource Type:: Dissertation
Full Text:: Philosophy Biochemistry and Biophysics presented on April 0, 1987 Title: THE BINDING OF ETHIDIUM BROMIDE TO

Carnitine and derivatives in embryonic chick tissues

Creator:: Casillas, Edmund Rene
Abstract:: Carnitine, acetylcarnitine, and long-chain acylcarnitine concentrations were determined for heart, brain, and liver of chick embryos during various stages of development. The total carnitine concentration was approximately the same in all three organs and showed only small variations during development. Acetylcarnitine was not detected in any organ until the seventeenth...
Resource Type:: Dissertation
Full Text:: total NADH produced and that one mole of NADH is produced per mole of acetyl-CoA generated from

Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide

Creator:: Tiffany, Thomas Oliver
Resource Type:: Dissertation
Full Text:: cc/mole for ribonuclease-S and -45 ± 2 cc/mole for S-protein. The difference in these values is

Size criterion for intercalation of hydrocarbon in DNA

Creator:: Craig, Albert Morrie
Abstract:: The binding of fifteen hydrocarbons to native DNA has been investigated. The hydrocarbons were chosen to test a size criterion for hydrocarbon - DNA complexes. It has been found that hydrocarbons, which are small enough to intercalate between the base pairs of a distorted Watson - Crick model of DNA,...
Resource Type:: Masters Thesis
Full Text:: Hydrocarbons in Salmon Sperm DNA Solution Hydrocarbon 104x Moles Hydrocarbon per Mole of DNA Phosphate

Purification of a ribonucleic acid-dependent ribonucleic acid polymerase from leukemic cells

Creator:: Watson, Kenneth Fredrick
Abstract:: There is good evidence that the infection with avian myeloblastosis virus BAI strain A causes myeloblasts to retain their primitive nature. These cells are able to multiply and produce virus at a relatively constant rate. From these observations, a major problem develops in the study of the virus-induced leukemia, that...
Resource Type:: Dissertation
Full Text:: cells are able to multiply and produce virus at a rela- tively constant rate. From these observations

Accessibility of highly conserved RNA sequences of Escherichia coli 70S ribosomes and implications for developing antibacterial agents

Creator:: Rahman, Mohammad A.
Abstract:: Escherichia coli 16S-like ribosomal RNAs from eubacterial, archaebacterial and eukaryotic organisms have several universally conserved sequence zones which are proposed to be single-stranded. A clear function for all of these regions has not yet been defined. To evaluate the roles of these sequence zones in protein synthesis, knowledge of their...
Resource Type:: Dissertation
Full Text:: Escherichia coli 70S ribosomes and implications for developing antibacterial agents AN ABSTRACT OF THE

Biological activity of avian myeloblastosis virus RNA in cell-free systems for protein synthesis

Creator:: Olson, Kenneth Charles
Abstract:: Avian myeloblastosis virus RNA was fractionated into a high molecular weight RNA and a low molecular weight RNA fraction by sucrose density centrifugation. The larger RNA component had a sedimentation constant of 65S and the low molecular weight RNA about 4S. The low molecular RNA has two biological properties of...
Resource Type:: Masters Thesis
Full Text:: (Name of student) M. S. (Degree) in Biochemistry and Biophysics presented on 5 , - s- NC°1(0

Subunit structure of Cancer magister Hemocyanin

Creator:: Carpenter, Donald Ellis
Resource Type:: Dissertation
Full Text:: in Figure 8 from the M of a model calculated for M = 71,600 and B = 3.861 10-7 mole/gm-fringe. 39 11

Synthesis of Glutamic-γ-semialdehyde in Escherichia coli : isolation of a low molecular weight compound required for in vitro synthesis

Creator:: Hartz, Judith Ann
Abstract:: Preliminary work on a crude in vitro system for glutamic-γ-semialdehyde synthesis in Escherichia coli has been described. The cell extract synthesis of GSA is inhibited by proline; other allosteric characteristics observed include sigmoidal substrate variation and inhibition plots of activity. The membrane fraction and a low molecular weight compound in...
Resource Type:: Masters Thesis
Full Text:: activity. The membrane fraction and a low mole- cular weight compound in the soluble fraction were

A partial characterization of the deoxyribonucleic acid found in the thoracic muscles of the tobacco hornworm moth (Manducta sexta)

Creator:: Hinds, Thomas Richard, 1944-
Abstract:: The DNAs (deoxyribonucleic acids) of the thoracic tissue of the tobacco hornworm, Manducta sexta, were studied by physical and chemical means. The adult moth was considered to be fully formed two days after emergence from the pupal case, as indicated by its ability to fly and the integrity of the...
Resource Type:: Dissertation
Full Text:: nuclear and debris DNA from the tobacco hornworm. 99 6. Mole % GC for some insects. 101 7. Optical

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: hydroxymethylase has a high Km of 600 p.M (Pizer and Cohen, 1962) and a slow turnover of 276 moles hmdCMP/ mole

The effect of streptomycin on proline synthesis in Escherichia coli

Creator:: Couch, David Bruce
Abstract:: The effect of streptomycin on an early reaction of proline synthesis, the production of glutamic-γ-semialdehyde, in resting cell suspensions of antibiotic-sensitive, resistant, and dependent strains of Escherichia coli was examined, and the effect on glutamic-γ-semialdehyde production was found to correspond to the growth response of the organisms to streptomycin. Inhibition...
Resource Type:: Masters Thesis
Full Text:: . 5x 10 2 moles) NH4C1 was cautiously added, and stirring continued for an additional hour. The

A role for sterols as modulators of membrane fluidity

Creator:: McLean-Bowen, Colleen Ann
Resource Type:: Dissertation
Full Text:: of mitochondria] membrane preparations from mutant and wild type cells were probed using the

The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNA

Creator:: Almehdi, Mirza A.
Abstract:: Ribosomes are multicomponent macromolecular particles and are essential for the survival of cells in all organisms. The ribosome's universal function is to catalyze polypeptide synthesis through translation of mRNA transcripts. Ribosomes from Escherichia coli, eubacterial organisms, have a sedimentation coefficient of 70S and are composed of 30S and 50S ribonucleoprotein...
Resource Type:: Dissertation
Full Text:: The function and structural characteristics of conserved

The oxidative demethylation of certain N-methylhydrazines by microsomal liver enzymes

Creator:: Wittkop, Judith Anne
Abstract:: The presence in rat liver microsomes of an enzyme system which demethylates N-methylhydrazines and azo compounds to formaldehyde has been reported. Some of the compounds found to be substrates were monomethylhydrazine, N-isopropyl-α-(2-methylhydrazino)- p-toluamide and the azo derivative of the latter hydrazine. This enzyme can be classified as a mixed function...
Resource Type:: Dissertation
Full Text:: -methylhydrazines and azo compounds to for- maldehyde has been reported. Some of the compounds found to be

Reactions of horseradish peroxidase with the isothiocyanates of horseradish oil

Creator:: Darussamin, Asril
Abstract:: Horseradish peroxidase (HRP) has been investigated extensively and for many years. In spite of this it still exhibits unexplained anomalies. Among these is variation in the reported number of isozymes (from 7 to 42), and reports of instability of the isozyme patterns. Since horseradish is a rich source of organic...
Resource Type:: Dissertation
Full Text:: . Amino Acid and Carbohydrate Composition of Peroxidase Isozymes. Moles of amino acid or sugar per mole

Studies on sterol : UDP glucose glucosyltransferase in germinating pea seedling

Creator:: Fang, Ta-Yun
Abstract:: Steryl glycoside is synthesized from mevalonate [2- ¹⁴C] in both maturing pea seeds and etiolated pea seedlings. The maturing seed appeared more active than the seedlings for this synthesis. The sugar moiety was identified predominantly as glucose by acid hydrolysis and paper chromatography of the aqueous extract of the hydrolysate....
Resource Type:: Dissertation
Full Text:: activity (275 1.4Ci/mole) UDP-glucose-14C. The reaction was stopped and extracted with two volumes of

Trinitrophenylation of bovine muscle pyruvate kinase

Creator:: Johnson, Stephen Craig
Resource Type:: Dissertation
Full Text:: McQuate and Utter (1959), an apparent A G of -5.2 kcal/mole can be derived. Since the reaction is only

Allosteric properties and the association equilibria of hemocyanin from Callianassa californiensis

Creator:: Arisaka, Fumio
Abstract:: Monomer-tetramer association equilibrium and the oxygen binding of the hemocyanin from Callianassa californiensis were measured at a number of pH values and magnesium ion concentrations. Magnesium binding of the hemocyanin was also measured at several pH values. A thermodynamic model, which satisfied the constraints from the experimental results, was developed...
Resource Type:: Dissertation
Full Text:: of Doctor of Philosophy Biochemistry in and Biophysics presented on May 5, 1977 Title: Allosteric

Physico-chemical studies on the structure of eukaryotic chromatin

Creator:: Sahasrabuddhe, Chintaman Gopal
Abstract:: It has been shown that about 50 % of the whole chromatin DNA exists in a very compact form in calfthymus chromatin.These compact fragments can be extracted from the whole chromatin as nuclease resistant fragments by limited nuclease digestion. These fragments involve four out of five histones in folding the...
Resource Type:: Dissertation
Full Text:: SAHASRABUDDHE for the DOCTOR OF PHILOSOPHY (Name of student) ( Degree) in Biochemistry and Biophysics

Intercalation on hydrocarbons in polynucleotides

Creator:: Craig, Albert Morrie
Abstract:: The binding of eleven polycyclic, aromatic hydrocarbons to the acid form of poly A has been investigated. The hydrocarbons were chosen to test a size criterion. Model building showed that some hydrocarbons could intercalate in the helix and be well protected from contact with the aqueous solvent. Experimentally it was...
Resource Type:: Dissertation
Full Text:: helix and be well protected from contact with the aqueous solvent. Experimentally it was found that

In vivo and in vitro metabolism of 4-benzo(b)thienyl-N-methylcarbamate and 4-hydroxy benzothiophene by the white rat

Creator:: Miaullis, John Bartley
Abstract:: In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate) metabolism have demonstrated that at least three ether soluble metabolites are formed in a microsomal system fortified with reduced triphosphopyridine nucleotide (NADPH). One of the metabolites was identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam. The other two metabolites did not possess...
Resource Type:: Masters Thesis
Full Text:: In vivo and in vitro metabolism of 4-benzo(b)thienyl-N

In vivo and in vitro metabolism of 4-benzo(b)thienyl-N-methylcarbamate and 4-hydroxy benzothiophene by the white rat

Creator:: Miaullis, John Bartley
Abstract:: In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate) metabolism have demonstrated that at least three ether soluble metabolites are formed in a microsomal system fortified with reduced triphosphopyridine nucleotide (NADPH). One of the metabolites was identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam. The other two metabolites did not possess...
Resource Type:: Masters Thesis
Full Text:: In vivo and in vitro metabolism of 4-benzo(b)thienyl-N

Regulation of ribonucleotide reductase analyzed by simultaneous measurement of the four enzyme activities

Creator:: Hendricks, Stephen P.
Abstract:: The first committed step in DNA biosynthesis occurs by direct reduction of ribonucleotides. This reduction is catalyzed by ribonucleotide reductase (RNR), an enzyme which uses a unique radical mechanism to facilitate the transformation. All four DNA precursors are synthesized by a single enzyme. Therefore, an intricate pattern of regulation has...
Resource Type:: Dissertation
Full Text:: for the degree of Doctor of Philosophy in Biochemistry and Biophysics presented on March 12. 1998

Incorporation of chromatophores into multi-cellular biosensors

Creator:: Preston, R. Ryan
Abstract:: Methodologies that detect biologically active substances have important potential applications for medical diagnostics, drug discovery, and chemical and biological anti-terrorism efforts. The wide spectrum of potential analytes that induce a physiological response dictates that novel techniques be developed to more rapidly screen and characterize agents that are more economical and...
Resource Type:: Dissertation
Full Text:: degree of Doctor of Philosophy in Biochemistry and Biophysics presented on May 2, 2002. Title

Rapid kinetic studies of thymidylate synthase

Creator:: Mittelstaedt, Denice
Resource Type:: Dissertation
Full Text:: , Lewis et al., 1981 and Moore et al., 1984) have indicated only one mole of 2,-deoxynucleotide binds per

An electrophoretic investigation of nucleoprotein organization in yeast chromatin

Creator:: Szent-Györgyi, Christopher Stephan
Abstract:: I have investigated nucleoprotein organization in chromatin of the yeast, Saccharomyces cerevisiae. My approach has been to prepare insoluble, minimally per turbed chromatin from a mitochondrial DNA-free strain of yeast, using a number of new techniques, and then digest this substrate with micrococcal nuclease. The soluble products are electrophoresed on...
Resource Type:: Dissertation
Full Text:: about 2 moles Hl per mole nucleosomes (31). Glycerol has long been used to stabilize protein 12

Purification, characterization and comparative properties of hepatic microsomal mixed-function oxidase enzymes from rat and rainbow trout

Creator:: Williams, David Edward
Resource Type:: Dissertation
Full Text:: reviewed by Masters (57). Highly purified reductase from mammals contains one mole of both FAD and FMN per

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