Index Catalog // ScholarsArchive@OSU

Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances

Creator:: Dukovcic, Stephanie R.
Abstract:: Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE DISSERTATION OF Stephanie R. Dukovcic for the degree of Doctor of Philosophy

Partial purification and properties of a columnaricin isolated from the myxobacterium Chondrococcus columnaris

Creator:: Needleman, David Sol
Abstract:: Of the 14 strains of Chondrococcus columnaris examined, 4 produced antagonistic substances that inhibited the growth of other strains of this species. The antagonistic substance produced by strain 3-P63-12 was examined in greater detail. Since the inhibitory substance had an extremely narrow spectrum of activity, and was not transmissible in...
Resource Type:: Masters Thesis
Full Text:: inhibi- tory substance had an extremely narrow spectrum of activity, and was not transmissible in

Development of an enzyme-linked immunosorbent assay to detect antibodies against Bacillus thuringiensis subspecies israelensis in Mallard ducks (Anas platyrhynchos)

Creator:: Rutherford, Gregory J.
Abstract:: To develop an assay to detect antibodies to Bacillus thuringiensis subsp. israelensis in mallard ducks, a growth curve was first established for the bacterium. The growth curve indicated that the crystal delta endotoxin would be best harvested from the rest of the cell material after 12 hours of growth. The...
Resource Type:: Masters Thesis
Full Text:: Development of an enzyme-linked immunosorbent assay to

Rhizosphere microfloras associated with mycorrhizae of Douglas-fir and red alder

Creator:: Neal, John Lloyd
Abstract:: The mycorrhizal rootlets of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco.) and red alder (Alnus rubra Bong.) were extensively investigated. A jet-black mycorrhiza was found to be dominant on Douglas-fir rootlets. The fungal symbiont was identified as Cenococcum graniforme (Sow.) Ferd. and Winge. Two forms of mycorrhizae predominated on root systems of...
Resource Type:: Dissertation
Full Text:: presence of red alder nonmycorrhizal root sus- pension. An antibacterial substance, found in red alder

Lactic streptococci: growth characteristics and plasmid profiles of drug- and bacteriophage-insensitive mutants

Creator:: Wulf, John J.
Abstract:: Fast acid-producing streptomycin-resistant mutants for several strains of lactic streptococci currently used in commercial Cheddar cheese manufacture were isolated. Their isolation involved use of a recently developed differential plating medium that allows identification of fast acid-producing colonies. Both parents and mutants were characterized with regard to growth, acid production, proteolytic...
Resource Type:: Masters Thesis
Full Text:: storage abuse influenced the incidence of slow acid- producing variants in three selected strains of

Effect of mercury on β-galactosidase activity : possible relationship to lactose intolerance

Creator:: LaChance, Michele Collette
Abstract:: The ability of mercury to inhibit β - galactosidase activity from cell-free extracts of Streptococcus lactis, Escherichia colt and intestinal homogenates of hogs, female rats, and humans was studied. Mercuric acetate was added in vitro to all sources of β - galactosidase except for the rats, which were placed on...
Resource Type:: Masters Thesis
Full Text:: Toxicity of Mercury and Derivative Compounds 7 Inorganic Mercury Organic Mercury 8 Diagnosis 9 Treatment

Characterization of interferon and retroposon-like repetitive elements in salmonid fish

Creator:: Tengelsen, Leslie A.
Abstract:: Hatchery-reared salmonid fish routinely encounter stress due to handling, barging, tagging, and overcrowding. It has been demonstrated that there exists a direct correlation between stress and transient immune suppression which can last for many days in fish. Epizootic viral infections routinely appear in hatcheries and can have a devastating effect...
Resource Type:: Dissertation
Full Text:: describe the molecular and biological features of a salmonid IFN-like substance. The ultimate goal of

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

Creator:: Fuller, David Wallace
Abstract:: The purpose of this study was to find what effect temperature and immunization has on the phagocytic activity of fish leukocytes against certain disease causing bacteria. Also proposed was the isolation and characterization of a leukocytolytic factor produced by one of these bacteria, Aeromonas salmonicida. Investigations revealed that temperature had...
Resource Type:: Dissertation
Full Text:: . salmonicida produced more of this substance than an avirulant strain. This material was demon- strated to be

The association of DNA with other cellular materials in Escherichia coli lysates

Creator:: Porter, Bruce Wallace
Abstract:: The association of other materials with DNA has been studied extensively in higher organisms and more recently in bacteria. However, investigation of these complexes in bacteria have for the most part been restricted to the study of a specific function this association may serve, or a specific kind of association....
Resource Type:: Dissertation
Full Text:: occurs in the cell prior to lysis and that sonication will remove DNA from this complex. Treatment of

Metabolism of aroma bacteria as related to diacetyl production

Creator:: Brown, Ruth Elaine
Abstract:: Factors influencing diacetyl production by the aroma bacteria Leuconostoc citrovorum and Streptococcus diacetilactis were investigated. When grown in association with lactic streptococci, L. citrovorum strain 91404 decreased in cell numbers from 10⁸ to 10⁶ over two weeks of daily subculturing in sterile non-fat milk incubated at 21°C. The ability to...
Resource Type:: Masters Thesis
Full Text:: affected. Treatment of the milk with 0.03% H2O2 and sufficient 0.004% catalase to decompose the H2O2

ScholarsArchive@OSU

Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances

Partial purification and properties of a columnaricin isolated from the myxobacterium Chondrococcus columnaris

Development of an enzyme-linked immunosorbent assay to detect antibodies against Bacillus thuringiensis subspecies israelensis in Mallard ducks (Anas platyrhynchos)

Rhizosphere microfloras associated with mycorrhizae of Douglas-fir and red alder

Lactic streptococci: growth characteristics and plasmid profiles of drug- and bacteriophage-insensitive mutants

Effect of mercury on β-galactosidase activity : possible relationship to lactose intolerance

Characterization of interferon and retroposon-like repetitive elements in salmonid fish

Isolation and characterization of a leukocytolytic factor from Aeromonas salmonicida

The association of DNA with other cellular materials in Escherichia coli lysates

Metabolism of aroma bacteria as related to diacetyl production

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