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The isolation, comparison, and attenuation of several viruses infecting Oregon salmonids

Creator:: McMichael, John Seiple
Abstract:: The Oregon strain of the agent of infectious hematopoietic necrosis (IHN) was isolated from fingerling sockeye salmon (Oncorhynchus nerka) by J. L. Fryer in 1958. Before the study reported herein no other virus had been recovered from any fish population in Oregon. Therefore, in July, 1971 a survey was initiated...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE THESIS OF JOHN SEIPLE MCMICHAEL for the

Adaptation of molecular filtration for recovery of fish viruses from water

Creator:: Watanabe, Robin Aiko
Abstract:: The effectiveness of tangential flow filtration (TFF) for recovery of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) from large volumes of water was evaluated. In laboratory studies, virus recovery from IHNV-seeded water following concentration by TFF was approximately 13%. However, addition of 0.1 and 1% FBS...
Resource Type:: Masters Thesis
Full Text:: Recovery of Fish Viruses from Water Abstract approved: Redacted for Privacy ci John S. Rohovec The

Strain differentiation and detection of infectious hematopoietic necrosis virus

Creator:: LaPatra, Scott Edward
Abstract:: The virulence of selected isolants of infectious hematopoietic necrosis virus (IHNV) were differentiated and correlated with electrophoretic mobilities of virion structural proteins by sodium dodecylsulfate polyacrylamide gel electrophoresis. A type 1 electropherotype of IHNV was more virulent for kokanee salmon (Oncorhynchus nerka) and a type 2 strain more virulent for...
Resource Type:: Dissertation
Full Text:: to John Cvitanich for the thoughtful discussions and continual encouragement. Thanks to Joe Groff

Morphologic, molecular and antigenic characteristics of Bacteroides nodosus

Creator:: Gradin, Joseph Lloyd
Resource Type:: Dissertation
Full Text:: also extended to my committee members: Dr. John L. Fryer, Dr. Stephen L. Kaattari, Dr. Donald E

Viruses infecting salmonid fishes from Oregon : A. The occurrence and distribution of infectious pancreatic necrosis virus. B. The development of an attenuated strain of infectious hematopoietic necrosis virus (IHNV) for immunization of salmonids

Creator:: Tebbit, Guy Lee
Abstract:: The occurrence and distribution of fish viruses in Oregon were determined by the examination of anadromous and fresh water salmonids. Infectious pancreatic necrosis virus (IPNV) was isolated and identified from asymptomatic adult coho (Oncorhynchus kisutch) and chinook (0. tshawytscha) salmon returning to the Columbia River system. Infectious pancreatic necrosis virus...
Resource Type:: Dissertation
Full Text:: Commission of Oregon, with special gratitude to Mr. Rich Holt and Mr. John Conrad; and the Oregon Wildlife

Distribution and epizootiology of infectious pancreatic necrosis and infectious hematopoietic necrosis of salmonid fishes in Oregon

Creator:: Mulcahy, Daniel M.
Abstract:: The distribution in Oregon of two viruses affecting salmonid fishes was determined by examination of hatchery and feral fish. Infectious pancreatic necrosis virus (IPNV) was isolated from fish at the Oregon Department of Fish and Wildlife Research Laboratory, Corvallis, Roaring River, Fort Klamath, and Wizard Falls Hatcheries. Fish collected at...
Resource Type:: Dissertation
Full Text:: McMichael (1974) isolated IHN virus and, for the first time in Oregon, infectious pancreatic necrosis (IPN

Molecular cloning of infectious pancreatic necrosis virus and characterization of the coding capacity of the complementary DNA

Creator:: Huang, Manley T. F.
Abstract:: The two segments of double-stranded RNA (dsRNA) from infectious pancreatic necrosis virus (IPNV) were cloned into plasmid vector pUC8. Two distinct sets of overlapping clones were identified by restriction enzyme and Southern blot analysis. Each of these sets was shown, by Northern blot analysis, to be exclusively related to either...
Resource Type:: Dissertation
Full Text:: the course of my program and who are cherished sources of knowlege and experience. To John Baross

Partial characterization of six established salmonid cell lines

Creator:: Philippon-Fried, Marie
Abstract:: Six salmonid cell lines previously established in this laboratory, CHSE 114 and CHSE 214 (chinook salmon), CSE 119 (coho salmon), SSE 5 (sockeye salmon), STE 137 (steelhead trout) and RTH 149 (rainbow trout), were partially characterized. Mycoplasmas were not detected in any of the cell lines. All cell lines exhibited...
Resource Type:: Masters Thesis
Full Text:: Rights Reserved ACKNOWLEDGEMENTS I wish to express my deepest gratitude to Drs. John L. Fryer and Jo

Recombinant vaccines against infectious hematopoietic necrosis virus : bacterial systems for vaccine production and delivery

Creator:: Simon, Benjamin E.
Abstract:: Several systems were examined for the production and delivery of recombinant vaccines for fish. C. crescentus was employed to produce a fragment of the IHNV glycoprotein. When administered by injection to 0.5 gram rainbow trout (Oncorhynchus mykiss), one of the fusion proteins (184 amino acids of the IHNV glycoprotein fused...
Resource Type:: Dissertation
Full Text:: challenging me to get to the basic "yes or no" questions. Thanks also to John Drennan, the best fisherman

Molecular cloning and analysis of the infectious hematopoietic necrosis virus genome and development of a subunit vaccine

Creator:: Gilmore, Robert D. (Robert Dean), 1956-
Abstract:: Complementary DNA (cDNA) clones were generated from the RNA genome of infectious hematopoietic necrosis virus (IHNV) by using random DNA oligomers to prime first strand synthesis. These clones were mapped to their respective locations on the genome and used to determine the nucleotide sequence of the nucleocapsid (N) gene and...
Resource Type:: Dissertation
Full Text:: to Dr. John Fryer and Dr. John Rohovec for lending an ear. Most of all, my appreciation goes to my

ScholarsArchive@OSU

The isolation, comparison, and attenuation of several viruses infecting Oregon salmonids

Adaptation of molecular filtration for recovery of fish viruses from water

Strain differentiation and detection of infectious hematopoietic necrosis virus

Morphologic, molecular and antigenic characteristics of Bacteroides nodosus

Viruses infecting salmonid fishes from Oregon : A. The occurrence and distribution of infectious pancreatic necrosis virus. B. The development of an attenuated strain of infectious hematopoietic necrosis virus (IHNV) for immunization of salmonids

Distribution and epizootiology of infectious pancreatic necrosis and infectious hematopoietic necrosis of salmonid fishes in Oregon

Molecular cloning of infectious pancreatic necrosis virus and characterization of the coding capacity of the complementary DNA

Partial characterization of six established salmonid cell lines

Recombinant vaccines against infectious hematopoietic necrosis virus : bacterial systems for vaccine production and delivery

Molecular cloning and analysis of the infectious hematopoietic necrosis virus genome and development of a subunit vaccine

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