Index Catalog // ScholarsArchive@OSU

Terminal methylation reactions in Saccharomyces cerevisiae

Creator:: Botsford, James Lawrence
Abstract:: A cell-free system for assessing the role of S-adenosyl-methionine in the methylation of homocysteine deriving the methyl group from serine has been developed. No role for S-adenosyl-methionine could be shown in the in vitro reaction The enzyme serine transhydroxymethylase has been studied in crude and partially purified cell extracts. Evidence...
Resource Type:: Dissertation
Full Text:: Terminal methylation reactions in Saccharomyces cerevisiae

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Creator:: Marshall, Nikki B.
Abstract:: The immune toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been studied for over 35 years, but only recently has the profound immune suppression associated with TCDD exposure been linked to induction of regulatory T cells (Tregs). The effects of TCDD are mediated through binding the aryl hydrocarbon receptor (AhR), a ligand-activated transcription...
Resource Type:: Dissertation
Full Text:: lymphocyte reaction. Antigen-processing appeared unaffected as phagocytosis of latex beads and antigen

Regulation of the terminal reactions for methionine biosynthesis in yeast

Creator:: Spence, Kemet Dean
Abstract:: Studies were carried out to elucidate the mechanism of enzymatic control of methionine biosynthesis in Saccharomyces cerevisiae. Enzymatic studies demonstrated that, in addition to the methionine activating enzyme, the S-adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in the presence of excess methionine. A similar, though reduced effect was observed when...
Resource Type:: Dissertation
Full Text:: Regulation of the Terminal Reactions For Methionine

Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

Creator:: Muslin, Omran
Abstract:: Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
Resource Type:: Masters Thesis
Full Text:: : Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column Abstract approved

Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water

Creator:: Brooks, Lauren
Abstract:: High counts of fecal indicators, used to signal the potential presence of pathogens associated with untreated waste, result in the classification of water bodies throughout the United States as impaired. Nonpoint sources of unknown origin that contribute to fecal contamination make management of impaired waters challenging, as they are difficult...
Resource Type:: Dissertation
Full Text:: Surface Water. Abstract approved: ______________________________________________________

Differential agar media for the identification of the Salmonella and Arizona groups of Enterobacteriaceae

Creator:: Stroup, John Raymond
Abstract:: Twelve differential agars have been studied in the development of media to presumptively identify the Salmonella Subgenera I-IV. Medium No. 7B/A (or Medium No. 7-H₂S Strip) combined the malonate, dulcitol, lysine decarboxylase and hydrogen sulfide reactions in one tube. It was designed to be used at the TSI stage of...
Resource Type:: Masters Thesis
Full Text:: reactions. However, it was noted that the hydrogen sulfide reaction was slower in the acid medium

Suppression of cellular immune responses by Candida albicans

Creator:: Rivas Espinoza, Victor G.
Abstract:: Experimental infection of mice with Candida albicans leads to significant suppression of cell mediated immune responses. We have characterized the nature of this candida-induced suppression with formalin killed Candida albicans (FKCA) in the yeast and mycelia' phase. Mice (BALB/c or C57B1/6J) treated with FKCA exhibited a reduction in the T-cell...
Resource Type:: Masters Thesis
Full Text:: response and in the mixed lymphocyte reaction. Further work has shown that the suppression is due to the

The cysteine to homocysteine conversion in Saccharomyces cerevisiae

Creator:: Sorsoli, Wayne Aldo
Abstract:: The mechanism of transsulfuration and the role of cystathionine in the biosynthesis of methionine in yeast were investigated. Saccharomyces were shown to accumulate cystathionine by use of the ³⁵S labeled compound. Cell-free extracts of the wildtype clone as well as methionine auxotrophs were shown to cleave cystathionine. The enzymatically produced...
Resource Type:: Dissertation
Full Text:: the enzymatic reaction mixture. The sulfhydryl was shown to be homocysteine by comparing the Rf

Toxigenic and non-toxigenic strains of Clostridium botulinum type E : A. Differentiation by immunodiffusion : B. Attempts to induce toxigenicity by mutagenic agents

Creator:: Balstraz, Edwina Gene
Abstract:: In this study of toxigenic and non-toxigenic strains of Clostridium botulinum type E, attempts were made to induce toxin production in non-toxigenic cultures by exposure to mutagenic agents and to differentiate the organisms by immunodiffusion. First, cultures of non-toxic Cl. botulinum type E were exposed to various mutagenic agents in...
Resource Type:: Masters Thesis
Full Text:: a single line precipitin reaction visible in a strong beam of light. These strains did not react

Partial purification and characterization of hemolysin from a psychrotrophic Kanagawa positive marine vibrio

Creator:: Huggins, Susan Peters
Abstract:: Psychrotrophic Kanagawa positive marine vibrios distinct from V. parahaemolyticus were isolated from shellfish collected in Yaquina Bay, Oregon. Sample enrichment in GSTB was followed by culture isolation on TCBS agar. The 235 vibrio isolates obtained were screened for gram reaction and morphology, Kanagawa reaction on Wagatsuma agar and their response...
Resource Type:: Masters Thesis
Full Text:: vibrio isolates obtained were screened for gram reac- tion and morphology, Kanagawa reaction on

ScholarsArchive@OSU

Terminal methylation reactions in Saccharomyces cerevisiae

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Regulation of the terminal reactions for methionine biosynthesis in yeast

Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water

Differential agar media for the identification of the Salmonella and Arizona groups of Enterobacteriaceae

Suppression of cellular immune responses by Candida albicans

The cysteine to homocysteine conversion in Saccharomyces cerevisiae

Toxigenic and non-toxigenic strains of Clostridium botulinum type E : A. Differentiation by immunodiffusion : B. Attempts to induce toxigenicity by mutagenic agents

Partial purification and characterization of hemolysin from a psychrotrophic Kanagawa positive marine vibrio

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